The principle of this kit is the reaction of formaldehyde with acetoacetanilide in the presence of ammonia. Neither a MESNA (4-Morpholineethanesulfonic acid, sodium) buffer nor EDTA in the test sample would be expected to interfere with this reaction. Having said that, it would be appropriate to run a blank containing MESNA and EDTA, and standards spiked with MESNA and EDTA, to confirm they they are not interfering with the test result...that is, making it either artificially high or low.
MAK131
Formaldehyde Assay
sufficient for 100 fluorometric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 fluorometric tests
detection method
fluorometric
relevant disease(s)
cancer
storage temp.
2-8°C
Related Categories
General description
Formaldehyde is widely employed in industry for a wide range of applications. It acts as a disinfectant and biocide, and is utilized as a tissue fixative and embalming agent.[1] Formaldehyde is a key metabolite produced during normal metabolism and contributes to methylation reactions and protein and nucleic acid synthesis. Increased production of formaldehyde has been observed in many cancer cell lines and increased formaldehyde concentration in urine has been associated with bladder and prostrate cancer.[2]
Application
- Modified Natural Rubber as a Simple Chemical Sensor with Smartphone Detection for Formaldehyde Content in a Seafood Sample.: This article explores the use of modified natural rubber as a chemical sensor, paired with smartphone detection, to measure formaldehyde content in seafood. This approach highlights the potential for accessible and mobile detection systems in food safety applications (Yeerum et al., 2022).
- Optical enzymatic formaldehyde biosensor based on alcohol oxidase and pH-sensitive methacrylic-acrylic optode membrane.: The study develops an optical enzymatic biosensor for formaldehyde, using alcohol oxidase with a pH-sensitive membrane, offering a novel approach for quick and sensitive detection in various analytical applications (Nurlely et al., 2022).
Features and Benefits
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.
Suitability
Suitable for the detection of formaldehyde in urine and other biological samples.
Principle
The Formaldehyde Assay kit provides a simple and direct procedure for measuring formaldehyde levels in urine and other biological samples. In this assay, formaldehyde is derivatized with acetoacetanilide in the presence of ammonia resulting in a fluorescent (λex = 370/λem = 470 nm) product, proportional to the formaldehyde present.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 1 - STOT SE 3
Target Organs
Eyes,Central nervous system, Respiratory system
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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Cristina A Nadalutti et al.
Scientific reports, 10(1), 5575-5575 (2020-03-30)
Formaldehyde (FA) is a simple biological aldehyde that is produced inside cells by several processes such as demethylation of DNA and proteins, amino acid metabolism, lipid peroxidation and one carbon metabolism (1-C). Although accumulation of excess FA in cells is
Formaldehyde in the workplace and in the home: exploring its clinical toxicology.
Montanaro A.
Laboratory Medicine, 27(11), 752-758 (2015)
Lysine-specific demethylase 1 in breast cancer cells contributes to the production of endogenous formaldehyde in the metastatic bone cancer pain model of rats.
Liu J, et al.
PLoS ONE, 8(3), e58957-e58957 (2013)
Wancang Liu et al.
Frontiers in microbiology, 11, 1988-1988 (2020-09-26)
Traditional diosgenin manufacturing process has led to serious environmental contamination and wastewater. Clean processes are needed that can alternate the diosgenin production. The β-glucosidase FBG1, cloned from Fusarium sp. CPCC 400709, can biotransform trillin and produce diosgenin. In this study
Wan-Cang Liu et al.
Microbial biotechnology, 12(6), 1226-1236 (2019-05-28)
Pichia pastoris KM71H (MutS ) is an efficient producer of hard-to-express proteins such as the membrane protein P-glycoprotein (Pgp), an ATP-powered efflux pump which is expressed properly, but at very low concentration, using the conventional induction strategy. Evaluation of different
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Is the assay compatible with MES buffer? Is EDTA affecting the reaction?
1 answer-
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What is the limit of detection that the MAK131 Formaldehyde Assay is capable of?
1 answer-
The kit is capable of detecting formaldehyde down to 1.5 micromolar or 45 ppb. The upper end of detection for undiluted samples is 100 micromolar, which is the highest concentration of the standard curve.
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