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MAK008

Sigma-Aldrich

Hydroxyproline Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Hydroxyproline Quantification Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

dermatological diseases

shipped in

wet ice

storage temp.

2-8°C

General description

Hydroxyproline (4-hydroxyproline) is a non-proteinogenic amino acid[1] formed by the post-translational hydroxylation of proline. Hydroxyproline is a major component of collagen, where it serves to stabilize the helical structure.[2] Because hydroxyproline is largely restricted to collagen, the measurement of hydroxylproline levels can be used as an indicator of collagen content. Conditions that increase collagen turnover can elevate serum and urine hydroxyproline levels. Urine and serum hydroxyproline levels can be used as a marker for bone resorption.[3][4]

Application

Hydroxyproline Assay Kit has been used to determine the concentration of hydroxyproline and collagen.[5][6][7]

Suitability

Suitable for hydroxyproline detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

Principle

In the Hydroxyproline Assay Kit, hydroxyproline concentration is determined by the reaction of oxidized hydroxyproline with 4-(Dimethylamino)benzaldehyde (DMAB), which results in a colorimetric (560 nm) product, proportional to the hydroxyproline present.

replaced by

Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Ox. Liq. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT RE 2 - STOT SE 3

Target Organs

Central nervous system, Thyroid

Supplementary Hazards

EUH031

Storage Class Code

5.1B - Oxidizing hazardous materials

Flash Point(F)

64.4 °F

Flash Point(C)

18 °C

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8L15K05550
8L14K05550
8J06K05550
7M14K05550
8H15K05550

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Gianluca Moroncini et al.
PloS one, 13(6), e0196048-e0196048 (2018-06-02)
Lung fibrosis is a severe condition resulting from several interstial lung diseases (ILD) with different etiologies. Current therapy of ILD, especially those associated with connective tissue diseases, is rather limited and new anti-fibrotic strategies are needed. In this study, we
Lodish H, et al.
Molecular Cell Biology. 4th edition. (2000)
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BioMed research international, 2015, 867140-867140 (2016-02-18)
Acute kidney injury (AKI) is associated with an increased risk of developing advanced chronic kidney disease (CKD). Yet, effective interventions to prevent this conversion are unavailable for clinical practice. In this study, we examined the beneficial effects of Tanshinone IIA
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Questions

1–10 of 13 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Where can I find the protocol for the hydroxyproline assay ?

    1 answer

    1. Please use the links below to access the protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/104/862/mak008pis-ms.pdf

      Available datasheets and protocols are typically located in the 'DOCUMENTATION' section under the 'More Documents' tab. Please see the link below to review this section of the product page:
      https://www.sigmaaldrich.com/product/sigma/mak008#product-documentation

      Helpful?

  4. Can this kit be used to quantify 3D collagen scaffolds or for coatings on scaffolds? Has it been done?

    1 answer

    1. This type of sample has not been tested with this assay kit. It is suggested to consult the scientific literature to determine if the specific sample type has been reported for this type of usage.

      Helpful?

  5. 1. Are heart samples that have been flash frozen suitable for use with MAK008-1KT?

    2. The manual mentions 10 mg of tissue, but does it need to be an exact measurement? Is it challenging to accurately weigh the frozen tissue?

    1 answer

    1. 1. Flash frozen tissue can be used with the kit. The recommended approach is to homogenize the tissue with room temperature water. Allow the tissue to thaw slightly by leaving it in the water for a few seconds before homogenization.

      2. The kit is optimized for use with 10 mg of tissue homogenate supernatant. Slight variations below or above 10 mg will not affect the assay significantly. However, it is advisable to avoid large differences in the weight or mass of the tissue.

      Helpful?

  6. Protocol recommends 10 mg tissue or cells for sample preparation. Is there a minimum quantity of cells required for this kit?

    1 answer

    1. It is recommended to start with 1-2 million cells per sample. However, determining a specific minimum amount is challenging because the level of hydroxyproline can vary significantly between different sample types.

      Helpful?

  7. Is it possible to carry out the 90-minute incubation at 60 °C, as mentioned in the protocol after adding the DMAB Reagent, using a hot plate? and, should the plate be covered during this incubation step?

    1 answer

    1. Yes, it is possible to perform the incubation on a hot plate. It is recommended to cover the plate during this process.

      Helpful?

  8. Is this kit suitable for tissue lysate samples?

    1 answer

    1. Yes, this kit is suitable for tissue lysate samples.

      Helpful?

  9. What is the expiration date of the product?

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  10. I want to study collagen production in the MRC-5 cell line under different experimental conditions, so I was wondering if I can use this assay kit to study the same?

    1 answer

    1. Yes, this kit is suitable for hydroxyproline detection, a major component of collagen. This kit is suitable for use in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

      Helpful?

1–10 of 13 Questions  

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