L9764
Lipopolysaccharides (rough strains) from Salmonella enterica serotype minnesota Re 595 (Re mutant)
Synonym(s):
LPS
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
biological source
Salmonella enterica (Serotype minnesota Re 595 mutant)
Quality Level
form
lyophilized powder
impurities
<3% Protein (Lowry)
color
white to yellow cast
solubility
water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow
shipped in
ambient
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
This product is prepared from Salmonella Minnesota strain Re 595 (Re mutant) using a phenol-chloroform-petroleum ether extraction procedure. The source strain is ATCC 49284. A unique structure in the LPS of strain Re 595 was shown to induce secretion and aggregation in human platelets.
Application
- Charge and aggregation pattern govern the interaction of plasticins with LPS monolayers mimicking the external leaflet of the outer membrane of Gram-negative bacteria: This study examines how charge and aggregation patterns affect the interaction of plasticins with lipopolysaccharides (LPS) from rough strains like Salmonella enterica serotype Minnesota Re 595. The findings contribute to understanding the molecular interactions involved in microbial resistance mechanisms (Michel et al., 2015).
Biochem/physiol Actions
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Preparation Note
Prepared by phenol-chloroform-petroleum ether extraction.
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Other Notes
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
related product
Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Tom C Freeman et al.
BMC biology, 10, 90-90 (2012-11-17)
This work describes the first genome-wide analysis of the transcriptional landscape of the pig. A new porcine Affymetrix expression array was designed in order to provide comprehensive coverage of the known pig transcriptome. The new array was used to generate
The impact of breed and tissue compartment on the response of pig macrophages to lipopolysaccharide.
Ronan Kapetanovic et al.
BMC genomics, 14, 581-581 (2013-08-30)
The draft genome of the domestic pig (Sus scrofa) has recently been published permitting refined analysis of the transcriptome. Pig breeds have been reported to differ in their resistance to infectious disease. In this study we examine whether there are
Julia Nagel et al.
Scientific reports, 8(1), 6204-6204 (2018-04-20)
Cholesteatoma is a potentially life-threatening middle ear lesion due to the formation of an inflamed ectopic mass of keratinizing squamous epithelium. Surgical removal remains the only treatment option, emphasizing the need to gain a better understanding of this severe disease.
G Banos et al.
BMC genomics, 18(1), 624-624 (2017-08-18)
Mastitis is the most prevalent disease in dairy sheep with major economic, hygienic and welfare implications. The disease persists in all dairy sheep production systems despite the implementation of improved management practises. Selective breeding for enhanced mastitis resistance may provide
Ronan Kapetanovic et al.
Journal of immunology (Baltimore, Md. : 1950), 188(7), 3382-3394 (2012-03-07)
Mouse bone marrow-derived macrophages (BMDM) grown in M-CSF (CSF-1) have been used widely in studies of macrophage biology and the response to TLR agonists. We investigated whether similar cells could be derived from the domestic pig using human rCSF-1 and
Articles
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service