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K0383

Sigma-Aldrich

Maleimide Activated KLH

Synonym(s):

Maleimide Activated Keyhole Limpet Hemocyanin

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About This Item

UNSPSC Code:
12352111
NACRES:
NA.32

biological source

Megathura crenulata

Quality Level

200

form

lyophilized powder

technique(s)

bioconjugation: suitable

storage temp.

2-8°C

General description

Maleimide Activated KLH is provided in lyophilized, ready to-use form, eliminating the need for time-consuming activation and purification steps of the carrier protein. Maleimide Activated KLH contains approximately 160-320 maleimide groups for each KLH molecule. The conjugation reaction with a cysteine containing peptide is performed at pH 6.6-7.0. The extent of conjugation can then be determined by a colorimetric assay with Ellman′s reagent to detect unreacted thiol groups in the cysteine-containing peptide.

Application

Maleimide Activated KLH has been used in peptide conjugation.
Maleimide-activated KLH (mKLH) has reactive maleimide groups on its surface, available for conjugation with a cysteine-containing peptide or a thiol-containing hapten. The reaction of the maleimide group and the thiol group proceeds rapidly and selectively under mild coupling conditions (pH 6.5-7.5) to yield a stable, covalently linked peptide-protein conjugate that may be used in immunization protocols or in antibody screening methods.

Physical form

Each vial contains 5 mg of Maleimide Activated KLH lyophilized from 10 mM sodium phosphate buffer, pH 6.6, with 0.115 mM NaCl, 1 mM EDTA, and 40 mM sucrose as stabilizer.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jannie Marie Sand et al.
PloS one, 8(12), e84934-e84934 (2014-01-01)
Fibrosis is characterized by excessive tissue remodeling resulting from altered expression of various growth factors, cytokines and proteases. We hypothesized that matrix metalloproteinase (MMP) mediated degradation of type IV collagen, a main component of the basement membrane, will release peptide
Jorma Isola et al.
International journal of molecular sciences, 24(13) (2023-07-14)
The enzyme transglutaminase 2 (TG2) plays a key role in celiac disease (CeD) pathogenesis. Active TG2 is located mainly extracellularly in the lamina propria but also in the villous enterocytes of the duodenum. The TG2 inhibitor ZED1227 is a promising

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