GE11-0032-75

HiTrap^® Capto^™ MMC

Cytiva 11-0032-75, pack of 5 × 5 mL

• UNSPSC Code: 23151817
• NACRES: NA.56

Properties

• description: Ion Exchanger Type (value)
• packaging: pack of 5 × 5 mL
• manufacturer/tradename: Cytiva 11-0032-75
• parameter: <20 ml/min flow rate; 42 psi
• bed size: 16 mm × 25 mm
• bed volume: 5 mL
• column I.D.: 16 mm
• matrix: highly cross-linked agarose
• avg. part. size: 75 μm (d50v)
• cleaning: 2-14
• working range: 2-12
• suitability: suitable for bioprocess medium

Description

General description

HiTrap^® Capto^™ MMC is prepacked with Capto^™ MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.

Application

The multimodal functionality gives a different selectivity compared with traditional ion exchangers including binding of Proteins regardless of ionic strength of the loading material . This means that the medium may be used for direct load of clarified feedstocks, without prior dilution to reduce the conductivity of the starting material. To reduce tedious sample preparation, the top filter in HiTrap^® Capto^™ MMC has been optimized for loading sonicated unclarified cell lysate directly on the column.

The Capto^™ MMC ligand may interact with Proteins in many different ways. It contains a carboxyl group, and thus it is a weak cation exchanger. However, in addition to the ionic interactions several other types of interactions are involved, including hydrogen bonding and hydrophobic interaction.

Features and Benefits

• High dynamic binding capacity at high conductivity.
• Selectivity allows binding in the presence of salt.
• High volume throughput.
• HiTrap^® Capto^™ MMC can be used to bind Proteins at the conductivity of the feed material and to solve specific purification challenges.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

Capto is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids