G7663
Gelatin blocking buffer
for Western blotting, powder blend
Synonym(s):
blocking buffer
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General description
Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).
Application
Suitable for use as a non-specific blocking agent for Western blots.
Reconstitution
Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.
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Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Protocols
In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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