F6636

Nutrient Mixture F-12 Ham

Coon′s Modification, with ʟ-glutamine and zinc sulfate, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): Ham’s F-12
• MDL number: MFCD00217417
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• product name: Nutrient Mixture F-12 Ham, powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, Coon′s Modification, suitable for cell culture
• form: powder
• technique(s): cell culture | hybridoma: suitable; cell culture | mammalian: suitable
• components: phenol red: 0.00125 g/L; L-glutamine: 0.292 g/L; sodium pyruvate: 0.22 g/L; glucose: 1.802 g/L
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

Coon's modification of Ham's F-12 was developed for culturing hybrid cells that were produced by viral fusion. The modification consists of doubling the amino acids and pyruvate and including ascorbic acid. The salt concentrations have also been altered. The formula contains 0.863 mg/L zinc sulfate, which may render it unsuitable for culturing mouse L-cells.

Quantity

Formulated to contain 11.5 grams of powder per liter of medium.

Reconstitution

Supplement with 2.68 g/L sodium bicarbonate.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves