Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.
SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets has been used in fluorescent in situ hybridization for the analysis of gene expression in neurons and synapses.[1][2]
Reconstitution
Each tablet set dissolved in 10 mL of deionized water yields a ready-to-use buffered solution.
In humans and other mammalian species, lesions in the preoptic area of the hypothalamus cause profound sleep impairment, indicating a crucial role of the preoptic area in sleep generation. However, the underlying circuit mechanism remains poorly understood. Electrophysiological recordings and
British journal of cancer, 80(3-4), 546-555 (1999-07-17)
The molecular mechanisms leading to androgen-independent growth in prostate cancer (PC) are poorly understood. Androgen deprivation therapy (ADT) results physiologically in a decrease in proliferation and an increase in programmed cell death (PCD)/apoptosis. The aim of our study was to
Journal of the science of food and agriculture, 92(15), 2944-2951 (2012-05-03)
Hordeins are major storage proteins of barley (Hordeum vulgare L.) grains and are considered to influence malting and brewing by forming a matrix surrounding the starch granules which affects the release of fermentable sugars. However, the extent to which environmental
Calcium imaging of basal forebrain activity during innate and learned behaviors
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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