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D5042

Sigma-Aldrich

123 bp DNA Ladder

for DNA electrophoresis

Synonym(s):

DNA Ladder for DNA electrophoresis, DNA marker

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About This Item

UNSPSC Code:
41105335
NACRES:
NA.25

Quality Level

200

form

liquid

usage

100 uses (0.1 mg size)
250 uses (250 μg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

Related Categories

Application

123 bp DNA Ladder has been used as a size marker in random amplified polymorphic DNA-polymerase chain reaction (PCR) analysis. It has also been used as a molecular marker in gel electrophoresis.

Components

Sigma′s 123 bp DNA Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, and 0.1 mM EDTA.

Other Notes

Contains 35 fragments, 123 - 4182 bp
For optimal resolution, the recommended agarose gel concentration is 1.5%.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J L Hartley et al.
Gene, 13(4), 347-353 (1981-05-01)
A method for self-ligation of DNA segments, which is based on the rotational non-equivalence of ends produced by AvaI cleavage, has been developed. Using this method and an initiator molecule to encourage the formation of long polymers, we have obtained
A simple and practical method that prepares high molecular weight DNA ladders
Zhang JH, et al.
Molecular Medicine Reports, 6(5), 1211-1213 (2012)
Molecular characterization of lactic acid bacteria isolated from beef and stored using vacuum-packaging and advanced vacuum skin packaging systems
Inguane AS, et al.
CYTA-journal of Food (2011)
Gene splicing and mutagenesis by PCR-driven overlap extension
Heckman KL and Pease LR
Nature Protocols, 2(4), 924-932 (2007)
Straightforward procedure for laboratory production of DNA ladder
Lan VTT, et al.
Journal of Nucleic Acids, 2012 (2012)
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Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

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Water for Nucleic Acid Gel Electrophoresis

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

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