product name
IMT 9, 95060901
biological source
deer skin
growth mode
Adherent
karyotype
Stable 2n = 6 genome
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Indian Muntjac skin fibroblast
Cell Line Description
The cell line IMT 9 has been derived from the non-transformed skin fibroblast cell line Indian Muntjac (Sigma product no. 90112813). Transformation was achieved by continuous exposure to low-level cadmium (5 μM) and a short non-toxic single dose of methyl-nitroso urethane (MNU, 24 h at 0.1μm). Exposure to cadmium led to the establishment of SCR5 (ECACC product no. 95071120); the MNU treatment of SCR5 cells followed by tumour formation in a nude mouse gave rise to the cell line IMT9. IMT9 is highly tumourigenic whereas SCR5 expresses a low tumour level. The cells have been eradicated from mycoplasma at ECACC.
Culture Medium
MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Dr. M. Ord, University of Southampton, Department of Biology, School of Biological Sciences, Southampton, UK.
Certificates of Analysis (COA)
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