OX-42 Cell Line from murine myeloma
Reactivity: Anti- CD11, rat, 87081803
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About This Item
UNSPSC Code:
41106514
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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
NS0/U
Reactivity
Anti-CD11, rat
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse NS0/U x Mouse BALB/c spleen
Cell Line Description
The antibody detects rat CD11. All peritoneal macrophages and 35% of alveolar macrophages react. The antibody affects the function of complement receptor CR3. It immunoprecipitates 3 polypeptides: 160kDa, 130kDa, 95kDa. The antibody also reacts with granulocytes and dendritic cells.
Immunogen
Resident peritoneal cells from (PVG-RT1c x PVG RT1u)F1 and (PVG-RT1c x DA-RT1a)F1 rats
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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