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50980

Sigma-Aldrich

Guanidine thiocyanate

BioUltra, for molecular biology, ≥99.0% (AT)

Synonym(s):

Guanidinium rhodanide, Guanidinium thiocyanate

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About This Item

Linear Formula:
NH2C(=NH)NH2 · HSCN
CAS Number:
593-84-0
Molecular Weight:
118.16
Beilstein:
3563461
EC Number:
209-812-1
MDL number:
MFCD00013027
UNSPSC Code:
12352107
PubChem Substance ID:
57651290
NACRES:
NA.25

grade

for molecular biology

Quality Level

100

product line

BioUltra

Assay

≥99.0% (AT)

form

solid

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

ign. residue

≤0.5% (as SO4)

loss

≤0.1% loss on drying, 20 °C (HV)

pH

4.5-7.0 (25 °C, 6 M in H2O)

mp

117-120 °C
120-122 °C (lit.)

solubility

H2O: 6 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤5000 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤5000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

absorption

cut-off at 280 nm in H2O at 6 M

SMILES string

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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Application

Chaotropic agent and strong denaturant; solubilizes cells.

Other Notes

Isolation of RNA; A new guanidine thiocyanate procedure for the isolation of full-length RNA templates for reverse transcription from tissues rich in RNase and proteoglycans

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

Supplementary Hazards

EUH032

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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J C Groppe et al.
Analytical biochemistry, 210(2), 337-343 (1993-05-01)
RNA isolated by conventional guanidinium isothiocyanate methods from tissues of a mollusc (red abalone: Haliotis rufescens) is largely degraded and discolored by contaminants. These contaminants are associated with inhibition of reverse transcriptase, prevent accurate spectrophotometric determination of RNA concentration, and
P Chomczynski et al.
Analytical biochemistry, 162(1), 156-159 (1987-04-01)
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is
Turpen, T.H. and Griffith, O.M.
Biotechniques, 4, 11-11 (1986)
Olesya V Stepanenko et al.
PloS one, 7(11), e48809-e48809 (2012-11-13)
Having a high folding efficiency and a low tendency to aggregate, the superfolder GFP (sfGFP) offers a unique opportunity to study the folding of proteins that have a β-barrel topology. Here, we studied the unfolding-refolding of sfGFP that was induced
Ling-Wen Ding et al.
Analytical biochemistry, 374(2), 426-428 (2008-01-02)
The most commonly used protocol of the RNA isolation, the guanidine thiocyanate method, was unsuitable for recalcitrant plant tissues containing a large amount of storage proteins and secondary metabolites. We demonstrated that RNA could bind to the silica particles, which
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