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Sigma-Aldrich

Atto 590-Biotin

BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

Synonym(s):

Biotin-Atto 590

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About This Item

Empirical Formula (Hill Notation):
C52H65ClN6O10S
Molecular Weight:
1001.62
MDL number:
MFCD04040677
UNSPSC Code:
12352108
NACRES:
NA.32

product line

BioReagent

Assay

≥90.0% (HPLC)

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 593-599 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Atto 590 is a new label with high molecular absorption (120.000) and quantum yield (0.80) as well as sufficient stoke′s shift (excitation maximum 594 nm, emission maximum 624 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Biotin conjugates can be used in applications like ELISA or immunohistochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Atto 590 NHS ester suitable for fluorescence, BioReagent, ≥90.0% (degree of coupling)

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Atto 590 NHS ester

Mariela M Marani et al.
Journal of combinatorial chemistry, 11(1), 146-150 (2008-12-17)
To screen one-bead-one-compound (OBOC) combinatorial libraries, tens of thousands to millions of compound beads are first mixed with a target molecule. The beads that interact with this molecule are then identified and isolated for compound structure determination. Here we describe
Shira Halivni et al.
ACS nano, 6(3), 2758-2765 (2012-02-09)
Fluorescence resonance energy transfer (FRET) involving a semiconductor nanoparticle (NP) acting as a donor, attached to multiple acceptors, is becoming a common tool for sensing, biolabeling, and energy transfer applications. Such nanosystems, with dimensions that are in the range of
D Wildanger et al.
Journal of microscopy, 236(1), 35-43 (2009-09-24)
The advent of supercontinuum laser sources has enabled the implementation of compact and tunable stimulated emission depletion fluorescence microscopes for imaging far below the diffraction barrier. Here we report on an enhanced version of this approach displaying an all-physics based
Andrea D Lehmann et al.
Small (Weinheim an der Bergstrasse, Germany), 6(6), 753-762 (2010-03-06)
Iron-platinum nanoparticles embedded in a poly(methacrylic acid) (PMA) polymer shell and fluorescently labeled with the dye ATTO 590 (FePt-PMA-ATTO-2%) are investigated in terms of their intracellular localization in lung cells and potential to induce a proinflammatory response dependent on concentration
Ronit Freeman et al.
Nano letters, 10(6), 2192-2196 (2010-05-21)
Semiconductor quantum dots (QDs) are used for the optical analysis of casein kinase (CK2) or the hydrolytic activity of alkaline phosphatase (ALP). Two schemes for the analysis of CK2 by a FRET-based mechanism are described. One approach involves the CK2-catalyzed
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