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22091

Millipore

Tryptic Soy Agar - Dehydrated Culture Media

NutriSelect® Plus, powder, suitable for microbiology

Synonym(s):

CASO Agar, Soybean Casein digest Agar, TSA, Tryptone Soy Agar, CASO Agar, Soybean Casein digest Agar, TSA, Tryptone Soya Agar

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About This Item

UNSPSC Code:
41171605
NACRES:
NA.74

Agency

according to GB 4789.40-2016
according to ISO 11731:2017
according to ISO 22964:2017

Quality Level

200

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
casein peptone (pancreatic), 15 g/L
sodium chloride, 5 g/L
soya peptone (papainic), 5 g/L

packaging

bottle of 2.5 kg
bottle of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

color

light yellow

final pH

7.3±0.2 (25 °C)

application(s)

agriculture
bioburden testing
cleaning products
clinical testing
cosmetics
environmental
food and beverages
personal care
surface monitoring
veterinary
microbiology

suitability

Enterococcus spp.
Haemophilus spp.
Pneumococcus spp.
nonselective for Bacillus spp.
nonselective for Bacteroides spp.
nonselective for Campylobacter spp.
nonselective for Candida spp.
nonselective for Cronobacter spp.
nonselective for Escherichia coli
nonselective for Neisseria spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

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General description

Tryptic Soy Agar is a general-purpose culture medium for the isolation and cultivation of fastidious and non-fastidious microorganisms or the maintenance of stock culture. It is a complex medium for the cultivation and isolation of a wide range of bacteria, yeasts, and molds. The formulation of the TSA medium contains a combination of casein and soy peptones supplying organic nitrogen, particularly amino acids, and longer-chained peptides. Sodium chloride maintains the osmotic equilibrium, and the natural sugars from soy peptone are the energy source.

Application

Tryptic Soy Agar is used for the pre-cultivation and enumeration (E. coli) by membrane filtration technique. It is suitable for the cultivation of both aerobic and anaerobic bacteria. Since it does not contain the X and V factors, it is suitable for the identification of Haemophilus sp. by adding X (Hemin) and V (DPN=NAD) factors strips.

Features and Benefits

NutriSelect® Plus are powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

Preparation Note

Suspend 40 g of dehydrated media in 1 litre of purified filtered water. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCL9639
BCCM3710
BCCK1970
BCCK7835
BCCL1557

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Byron Brook et al.
Science translational medicine, 12(542) (2020-05-08)
Death from sepsis in the neonatal period remains a serious threat for millions. Within 3 days of administration, bacille Calmette-Guérin (BCG) vaccination can reduce mortality from neonatal sepsis in human newborns, but the underlying mechanism for this rapid protection is
Mariana Lanzarini-Lopes et al.
Water research, 184, 116191-116191 (2020-07-30)
This paper investigates using UV-C side-emitting optical fibers (SEOFs) to prevent growth of pathogenic bacteria (Pseudomonas aeruginosa and Escherichia coli) on nutrient-rich surfaces. Attaching a SEOF to a single 265 nm light emitting diode (LED) increases irradiation area by >1000x
Binbin Deng et al.
iScience, 23(2), 100827-100827 (2020-02-15)
Pseudomonas aeruginosa biofilms represent a major threat to health care. Rugose small colony variants (RSCV) of P. aeruginosa, isolated from chronic infections, display hyperbiofilm phenotype. RSCV biofilms are highly resistant to antibiotics and host defenses. This work shows that RSCV
Tara R Catron et al.
Scientific reports, 9(1), 7064-7064 (2019-05-10)
Estrogenic chemicals are widespread environmental contaminants associated with diverse health and ecological effects. During early vertebrate development, estrogen receptor signaling is critical for many different physiologic responses, including nervous system function. Recently, host-associated microbiota have been shown to influence neurodevelopment.
Qian Du et al.
Journal of immunology (Baltimore, Md. : 1950), 201(2), 533-547 (2018-06-03)
Porcine circovirus (PCV) type 2 (PCV2), an immunosuppression pathogen, is often found to increase the risk of other pathogenic infections. Yet the relative immune mechanisms determining the susceptibility of PCV2-infected animals remain unclear. In this study, we confirmed that PCV2
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