11426320001
Roche
Anti-Fluorescein
from mouse IgG1 (clone B13-DE1)
Synonym(s):
antibody
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About This Item
UNSPSC Code:
12352200
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
B13-DE1, monoclonal
form
lyophilized
packaging
pkg of 100 μg
manufacturer/tradename
Roche
isotype
IgG1
storage temp.
2-8°C
General description
Monoclonal antibody to fluorescein.
The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate.
The antibody does not contain any protein and can hence be used for coating and labeling.
Contents
Lyophilizate, stabilized
The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate.
The antibody does not contain any protein and can hence be used for coating and labeling.
Contents
Lyophilizate, stabilized
Specificity
The monoclonal antibody reacts with free and bound fluorescein.
Application
Use Anti-Fluorescein for the detection of fluorescein-labeled compounds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization[1]
- Western blot
Preparation Note
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: Phosphate buffered saline, pH 7.4
Using water instead the antibody can precipitate.
- ELISA: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 2 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Working solution: Phosphate buffered saline, pH 7.4
Using water instead the antibody can precipitate.
Reconstitution
Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Reconstitute for 15 minutes.
Reconstitute for 15 minutes.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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