10837059001

Lysozyme

from hen egg white

• Synonym(s): Mucopeptide N-acetylmuramoyl-hydrolase, Muramidase, lysozyme
• Enzyme Commission number: 3.2.1.17 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: chicken egg white
• Quality Level: 100
• Assay: ≥95%
• form: solid
• specific activity: >23000 U/mg (Shugar units)
• mol wt: 14.4 kDa
• packaging: pkg of 10 g
• manufacturer/tradename: Roche
• optimum pH: 6.0-7.0
• absorption: 3.9 at 280 nm (10 mg enzyme/ml)

Description

General description

Muramidase, Mucopeptide N-acetylmuramoyl-hydrolase

Application

Lysozyme has been used for:

• Cell wall degradation[1]
• Preparation of protoplasts[2]
• Bacteriolysis[3]
• Pharmacology (anti-inflammatory effect on mucous membrane; healing of tissue; protection against bleeding; normalization of mucus secretion; dissolution of pyrogens; potentiation of antibiotics)
• Food and drinks (flavor enhancer)
• Sample preparation prior to isolation of nucleic acids[4]

Lysozyme has been used in the monitoring of transcription by real-time -PCR[5] and sample preparation.[6]

Biochem/physiol Actions

Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.[7]

Unit Definition

One Shugar unit is that quantity of enzyme in 1 ml of a suspension of Micrococcus luteus of pH 7.0 whose initial absorbance at a wavelength of 450 nm is 0.750 for a pathlength of 10 mm which causes the absorbance to decrease at the rate of 0.001 per minute, all measurements being carried out at a temperature of +25 °C.

Preparation Note

Working solution: Lysozyme hydrochloride is readily soluble in water and buffer solutions but insoluble in organic solvents.
Storage conditions (working solution): -15 to -25 °C
Aqueous solutions of the hydrochloride (2 mg/ml dist. water) can be stored for several days at 2 to 8° C or at -15 to -25° C for several weeks.

Reconstitution

Prepare a stock solution at a concentration of 50 mg/ml in dist. water. Dispense into aliquots and store at -15 to -25 °C. Discard each aliquot after use.

Storage and Stability

Store at 2 to 8 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): does not flash
• Flash Point(C): does not flash