The maximum size of cell clumps that can pass through the Scepter 3.0 is determined by the aperture limits, which are 40um and 60um for sensors 40 and 60, respectively. Unlike the Scepter 2.0, the Scepter 3.0 does not use a screen to break up cell clump aggregates; instead, it employs a set of pillars to achieve this function. In contrast, the Scepter 2.0 utilizes a 35um Nylon filter to break up cell clump aggregates.
PHCC360KIT
Scepter 3.0 Handheld Automated Cell Counter
Scepter 3.0 Handheld Automated Cell Counter
Rapid cell counts in a handheld easy to use format, includes pkg of 60 μm Scepter 3.0 Cell Counter Sensors
Synonym(s):
Cell counting instrument
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About This Item
UNSPSC Code:
41131501
NACRES:
NC.56
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manufacturer/tradename
Scepter
technique(s)
cell counting: suitable
particle size
6-36 μm
compatibility
for use with cells sized 4.0–25.0 μm
shipped in
ambient
General description
The Scepter 3.0 Handheld Automated Cell Counter delivers rapid cell counts in a handheld, easy to use format. Intended for research use only, Scepter 3.0 has been tested with cell types representative of those in use today. After preparing a dilution of the cell culture of interest, on-screen instructions guide the user through the sampling process. In less than 30 seconds after the Scepter Cell Counter aspirates a sample into the Scepter Cell Counter Sensor, the Scepter cell counter′s screen displays:
The Scepter 3.0 Cell Counter is compatible with both the 40uM and 60uM Scepter 3.0 Cell Counter Sensors. This kit (PHCC360KIT) includes a free 50-pack of 60uM sensors.
- Cell concentration
- Average cell size
- Average cell volume
- Histogram of size or volume distribution
The Scepter 3.0 Cell Counter is compatible with both the 40uM and 60uM Scepter 3.0 Cell Counter Sensors. This kit (PHCC360KIT) includes a free 50-pack of 60uM sensors.
Components
- 1 Scepter 3.0 Automated Handheld Cell Counter
- 1 Test Bead Vial
- 1 Charging Station
- 50 Sensors (60μM)
Other Notes
The Scepter 3.0 Cell Counter is not compatible with previous generation sensors; only compatible with Scepter 3.0 Cell Counter Sensors shown in the Related Products.
accessory
Product No.
Description
Pricing
Storage Class Code
10-13 - German Storage Class 10 to 13
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Data for validated human cell lines using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.
The Scepter™ 3.0 Cell Counter, when used in conjunction with a 40 μm aperture sensor, can accurately count and precisely size a wide range of cell types and particles. This application note demonstrates two examples of experiments using the instrument’s sensitive size-discriminating capacity for rapid qualitative assessment of individual cell populations.
Find answers to frequently asked questions about the Millicell® ERS 3.0, from probe depth adjustment to instrument maintenance and troubleshooting.
Learn how Sterivex™ pressure-driven sterile filters can be used for large-volume sample processing in environmental research and testing.
Protocols
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Related Content
Monitor your cell cultures with ease with the Millicell® DCI Digital Cell Imager. From confluency to cell count and morphology, the Millicell® DCI automates cell culture measurements for quicker, more consistent culturing.
Scepter™ Cell Counter is a portable device which brings consistency in cell counting right to the culture hood in less than 30 seconds.
A list of which mammalian and other key phenotypes have been validated for Scepter™ counting precision.
Scepter™ counting is 7 to 10 times faster than hemocytometry-and also faster than other automated counters. Learn more about how this amazing technology works
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What is the maximum size of cell clumps that can pass through the Scepter 3.0? Also, what size screen is used to break up the cell clump aggregates in both Scepter 2.0 and 3.0?
1 answer-
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Is this appropriate for size 15-23 μm cell? Does the Kit include also the charger? How many 60/40 μm sensors are included Which sensors can be used with this instrument? Which sensors are appropriate for cells size 15-23 μm and what is their price?
1 answer-
Please see the answers to the following questions below:
1. Is this appropriate for size 15-23 μm cells?
Yes, the Scepter 3.0 is suitable. As listed on the product detail page in the DESCRIPTION section for use with cells sized 4.0–25.0 μm.2. Does the Kit include the charger?
This product does include 60μM sensors and a charging station.3. How many 60/40 μm sensors are included
As mentioned on the product detail page in the DESCRIPTION section under 'Components', it includes 50 Sensors.4. Which sensors can be used with this instrument?
This product is only compatible with Scepter 3.0 Cell Counter Sensors shown in the Related Products on this page. Please see the link below to review additional information regarding the Scepter 3.0:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-counting-and-health-analysis/scepter-cell-counter5. Which sensors are appropriate for cells size 15-23 μm and what is their price?
See question 1, this product is suitable for the cell size of 15-23 μm.Regarding pricing, Please navigate to the link https://www.sigmaaldrich.com/support/customer-support, and click on "Products Pricing & Availability" under the Products Section with all the required information so that a member of the Customer Service team can reach out to assist further. Thank you.
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Does it require Trypan blue or another dye? Does it distinguish between live and dead cells?
1 answer-
The Scepter is a particle counter, but it will not differentiate live from dead cells. The device can be used to gauge cell health on the basis of cell size. For example, in cell types where apoptotic cells are smaller in size compared to non-apoptotic cells. This shift in size can be quantified using the device option "Edit Histogram" to either gate around this population or exclude the population from the count.
Please see the following manual below for more information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/799/484/00110230w-rev1020-ms.pdfHelpful?
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