Metabotropic glutamate receptor 5 (mGluR5) is a member of the G-protein coupled receptor family and functions as a receptor for glutamate. mGluR5 activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.
Immunogen
GST-tagged recombinant protein corresponding to human mGluR5.
Application
Detect mGluR5 using this mouse monoclonal antibody, Anti-mGluR5 Antibody, clone 11D9.1 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected mGluR5 in normal human brain tissue.
Quality
Evaluated by Western Blotting in rat brain micropreparation tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected mGluR5 in 10 µg of rat brain micropreparation tissue lysate. The lysate was not boiled during preperation for western blotting.
Target description
~150 kDa observed. The calculated molecular weight is 130 kDa, however mGluR5 has been shown as a ~150 kDa band in western blots (Romano, C., et al. (1996). J Biol Chem. 271(45):28612-28616).
Physical form
Format: Purified
Analysis Note
Control Rat brain micropreparation tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Journal of psychiatry & neuroscience : JPN, 46(1), E1-E13 (2020-06-20)
Glutamate transmission is implicated in drug-induced behavioural sensitization and the associated long-lasting increases in mesolimbic output. Metabotropic glutamate type 5 (mGlu5) receptors might be particularly important, but most details are poorly understood. We first assessed in mice (n = 51
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