MABN1152

Anti-UBE4B, clone EPR7471, Rabbit Monoclonal Antibody

clone EPR7471, from rabbit

• Synonym(s): Ubiquitin conjugation factor E4 B, Homozygously deleted in neuroblastoma 1, Ubiquitin fusion degradation protein 2, UBE4B, HDNB1, UFD2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: unpurified
• antibody product type: primary antibodies
• clone: EPR7471, monoclonal
• species reactivity: mouse, human, rat
• technique(s): western blot: suitable
• isotype: IgG
• UniProt accession no.: O95155
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... UBE4B(10277)

Description

General description

Ubiquitin conjugation factor E4 B, also known as Homozygously deleted in neuroblastoma 1 or Ubiquitin fusion degradation protein 2, and encoded by the gene name UBE4B or HDNB1 or UFD2, is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE4B is an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. It is also the strongest candidate in the neuroblastoma tumor suppressor.

Immunogen

Synthetic peptide corresponding to human UBE4B.

Application

Anti-UBE4B, clone EPR7471, Rabbit Monoclonal Antibody is an antibody against UBE4B for use in Western Blotting.

Produced in collaboration with Epitomics

Research Category
Neuroscience

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Quality

Evaluated by Western Blotting in HEK293, Raji, SK-OV-3, L929, L6, RAW264.7, PC12, NIH -T3, HeLa, and K562 cell lysates.

Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected UBE4B in HEK293, Raji, SK-OV-3, L929, L6, RAW264.7, PC12, NIH-3T3, HeLa, and K562 cell lysates.

Target description

~140 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Unpurified

Rabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2