MABE1990
Anti-PLZF/ZBTB16 Antibody, clone Mags-21F7
Synonym(s):
Promyelocytic leukemia zinc finger protein, Zinc finger and BTB domain-containing protein 16, Zinc finger protein 145, Zinc finger protein PLZF
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
hamster
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
Mags-21F7, monoclonal
mol wt
observed mol wt ~75 kDa
purified by
using protein G
species reactivity
human, mouse
packaging
antibody small pack of 100
technique(s)
flow cytometry: suitable
western blot: suitable
isotype
IgG2
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
Gene Information
human ... ZBTB16(7704)
Specificity
Clone Mags-21F7 is an Armenian Hamster monoclonal antibody that detects Zinc finger and BTB domain-containing protein 16 (PLZF/ZBTB16).
Immunogen
A combination of peptides covering the amino, carboxyl, and hinge regions of human Zinc finger and BTB domain-containing protein 16 (PLZF/ZBTB16).
Application
Quality Control Testing
Evaluated by Western Blotting with recombinant Human PLZF/ZBTB16 protein.
Western Blotting Analysis: A 1:500 dilution of this antibody detected full-length, FLAG-tagged recombinant Human PLZF/ZBTB16.
Tested Applications
Western Blotting Analysis: A representative lot detected PLZF/ZBTB16 in Western Blotting applications (Kovalovsky, D., et al. (2008). Nat Immunol. 9(9):1055-64).
Flow Cytometry Analysis: A representative lot detected PLZF/ZBTB16 in Flow Cytometry applications (Kovalovsky, D., et al. (2008). Nat Immunol.;9(9):1055-64; Kovalovsky, D., et al. (2010). J Immunol. 184(12):6746-55).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with recombinant Human PLZF/ZBTB16 protein.
Western Blotting Analysis: A 1:500 dilution of this antibody detected full-length, FLAG-tagged recombinant Human PLZF/ZBTB16.
Tested Applications
Western Blotting Analysis: A representative lot detected PLZF/ZBTB16 in Western Blotting applications (Kovalovsky, D., et al. (2008). Nat Immunol. 9(9):1055-64).
Flow Cytometry Analysis: A representative lot detected PLZF/ZBTB16 in Flow Cytometry applications (Kovalovsky, D., et al. (2008). Nat Immunol.;9(9):1055-64; Kovalovsky, D., et al. (2010). J Immunol. 184(12):6746-55).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Zinc finger and BTB domain-containing protein 16 (UniProt: Q05516; also known as Promyelocytic leukemia zinc finger protein, Zinc finger protein 145, Zinc finger protein PLZF) is encoded by the ZBTB16 (also known as PLZF, ZNF145) gene (Gene ID: 7704) in human. PLZF is a member of the krueppel C2H2-type zinc-finger protein family with nine C2H2-tpe zinc-finger domains. It serves as a transcriptional repressor and this repression may be mediated through recruitment of histone deacetylases to target promoters. It affects diverse signaling pathways, including growth-regulatory and differentiation pathways and is a key regulator of myeloid development. Its expression is observed in early, but in differentiated hematopoietic cells. It is expressed in bone marrow, early myeloid cells, and peripheral blood mononuclear cells. At lower level it is also detected in the ovary, kidney, and lung tissue. PLZF is reported to be essential for development of the characteristic innate/effector functions of natural killer T (NKT) cells. It is highly expressed during the differentiation of NKT cells and is essential for the acquisition of effector/memory innate-like functions. PLZF-deficient NKT cells lose their characteristic memory phenotype, and their function is significantly impaired. These cells lose their unique ability to co-secrete IL-4 and IFN- upon ex vivo stimulation. (Ref.: Kolesnichenko, M., and Vogt, PK. (2011). Cell Cycle.10(5); 771-775; Kovalovsky, D., et al. (2010). J. Immunol. 184: 6746-6755; Melnick, AM., et al. (2000). Mol. Cell Biol. 20(6); 2075-2086).
Physical form
Purified Armenian hamster monoclonal antibody IgG2-IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Reconstitution
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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