ELI-006-H
IL-5 ELISPOT Antibody Pair, Human
from rat, clone JES1-5A10, clone TRFK5
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
clone
JES1-5A10, monoclonal
TRFK5, monoclonal
species reactivity
human
technique(s)
ELISA: suitable
shipped in
wet ice
General description
Interleukin 5 (IL-5) is a hematopoietic growth factor that is primarily produced by activated T lymphocytes, and is approximately 70% homologically identical in both human and mouse. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, as well as stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. When production levels of this growth factor become elevated, IL-5 has also been shown to be linked to asthmatic conditions in both human and mouse models. The Millipore human IL-5 ELISPOT Antibody Pair provides a sensitive and highly reliable method for the detection of IL-5 secreting cells at the single cell level.
Specificity
Both the capture and the detection antibodies included in this kit react with human interleukin -5 (IL-5).
Application
IL-5 ELISPOT Antibody Pair, Human is a highly specific rat monoclonal antibody & that targets IL-5 & has been tested in ELISA.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Packaging
Sufficient reagents for five, 96-well plates.
Components
• Human IL-5 Capture Antibody, clone TRFK5. Quantity: 500 µg.
• Human IL-5 Detection Antibody, clone JES1-5A10. Quantity: 100 µg.
• Streptavidin Alkaline Phosphatase. Quantity: 55 μL.
• Human IL-5 Detection Antibody, clone JES1-5A10. Quantity: 100 µg.
• Streptavidin Alkaline Phosphatase. Quantity: 55 μL.
Target description
~26 kDa
Physical form
Format: Purified
The 500 µg purified IL-5 capture antibody is buffered in a preservative-free phosphate solution, pH 7.2, and should be handled under aseptic conditions. The 100 µg purified IL-5 biotinylated detection antibody is buffered in a phosphate solution, pH 7.2, containing 0.09% sodium azide. Streptavidin alkaline phosphatase is supplied in a Tris Buffer, pH 8.0, containing 0.1 M sodium chloride, 1 mM magnesium chloride, 0.2% (w/v) BSA, 10% (w/w) glycerol, and 0.1% sodium azide.
Storage and Stability
Maintain refrigerated at 2-8°C for up to six months from the date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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