A convenient kit for the identification of changes in the plasma membrane that occur during apoptosis. In apoptotic cells, phosphoatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, allowing for the detection of PS on the cell surface. In the presence of Ca 2+, Annexin V exhibits a high affinity for PS and binds cells with exposed PS. Annexin V, when conjugated to a fluorophore, can be used to monitor apoptopic cells using flow cytometry or immunofluorescence microscopy.
Components
Kit contains:
Annexin V-FITC
Binding Buffer
Propidium Iodide
user protocol.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
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