ABN304
Anti-Precerebellin Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Cerebellin precursor, Cerebellin, CER, Precerebellin
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
technique(s)
immunohistochemistry: suitable
western blot: suitable
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CBLN1(869)
General description
Precerebellin is the precursor of the brain-specific hexadecapeptide cerebellin, a protein with substantial similarity to the globular region of the B chain of complement component C1q. Cerebellin exerts neuromodulatory functions by directly stimulating norepinephrine release via the adenylate cyclase/pka-dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release. The active form of precerebellin is highly enriched in postsynaptic structures of cerebellar Purkinje cells in cartwheel neurons of the dorsal cochlear nucleus. Because precerebellin belongs to the C1q/tumor necrosis factor superfamily of secreted proteins and has similarity to adiponectin and CTRP3, it has been suggested that precerebellin possess functions other than those already stated.
Immunogen
KLH-conjugated linear peptide corresponding to human Precerebellin.
Application
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Precerebellin in human cerebellum tissue.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
This Precerebellin antibody is validated for use in WB & IHC for the detection of the Precerebellin protein.
Quality
Evaluated by Western Blot in mouse cerebellum lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Precerebellin in 10 µg/mL of mouse cerebellum tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Precerebellin in 10 µg/mL of mouse cerebellum tissue lysate.
Target description
~35 kDa observed. The calculated molecular weight is 22 kDa, however, the full length Precerebellin has been shown as a ~35 kDa band. (Bao, D., et al. (2005). Journal of Neurochemistry. 95:618-629.) An uncharacterized band may appear at ~50 kDa in some lysates.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse cerebellum tissue lysate
Mouse cerebellum tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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