AB356483

Anti-DsRed (RFP)

from rabbit

• Synonym(s): Red fluorescent protein drFP583:RFP
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity (predicted by homology): all (based on 100% sequence homology)
• packaging: antibody small pack of 25 μg
• technique(s): western blot: suitable
• isotype: IgG
• UniProt accession no.: Q9U6Y8
• target post-translational modification: unmodified

Description

General description

Red fluorescent protein drFP583 (UniProt: Q9U6Y8; also known as DsRed) is a fluorescent protein that serves as a useful and ubiquitous tool for making chimeric proteins, where it can function as a fluorescent protein tag. Fluorescent proteins serve as useful tools for making chimeric proteins and function as fluorescent protein tag. They can tolerate N- and C-terminal fusion to a broad variety of proteins. DsRed is a stable homotetrameric protein with high quantum yield and is derived from coral Discosoma. It is an 11-stranded beta-can with a central alpha-helix and its each monomer is structurally similar to GFP. DsRed has an orange-red fluorescence and is excited optimally at 558 nm, but can also be excited by a standard 488 nm laser, allowing it to be used with laser-based confocal microscopes and flow cytometers. DsRed offers stability to changes in external pH and its spectral properties are ideal for dual-color experiments with green fluorescent protein (Ref.: Bevis, BJ., and Glick, BS (2002). Nat. Biotech. 20; 83-87).

Specificity

This rabbit polyclonal antibody detects Red fluorescent protein drFP583. It targets an epitope within 6 amino acids from the N-terminal region.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to 6 amino acids from the N-terminal region of Red fluorescent protein drFP583 from Discosoma sp. (Sea anemone).

Application

Anti-DsRed (RFP) is a rabbit polyclonal antibody that detects Red fluorescent protein DsRed and has been tested for use in Western Blotting.

Research Category
Secondary & Control Antibodies

Western Blotting Analysis: A representative lot detected DsRed (RFP) in Western Blotting applications (Aihara, H., et. al. (2016). Mol Cell. 64(1):176-188).

Western Blotting Analysis: A 1:200 dilution of a representative lot detected DsRed (RFP) in whole cell lysate from 293T cell expressing HcRed & DsRed-mouse USP21 fusion protein. (Courtesy of Dr. Ito Takashi at Nagasaki University).

Quality

Evaluated by Western Blotting in whole cell lysate from 293T cell expressing DsRed-mouse USP21 fusion protein.

Western Blotting Analysis: 1 µg/mL of this antibody detected DsRed (RFP) in whole cell lysate from 293T cell expressing DsRed-mouse USP21 fusion protein.

Target description

~100 kDa observed; 25.93 kDA calculated. (Observed molecular weight includes USP21 (~63KDa). Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 50 mM KCL, 25 mM HEPES (pH 7.6), 10% glycerol, 0.1 mM EDTA, 0.01% NP40.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.