5.05759

MitoBloCK-6

• Synonym(s): MitoBloCK-6, 2-(((4-Anilinophenyl)imino)methyl)-4,6-dichlorophenol, 2,4-Dichloro-6-(((((phenylamino)phenyl)imino)methyl)phenol), Mitochondrial Protein Import Blocker from the Carla Koehler laboratory, MB6, MB-6
• Empirical Formula (Hill Notation): C₁₉H₁₄Cl₂N₂O
• CAS Number: 303215-67-0
• Molecular Weight: 357.23
• MDL number: MFCD00153366
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Assay: ≥98% (HPLC)
• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: orange
• solubility: DMSO: 100 mg/mL
• storage temp.: 2-8°C

Description

General description

A cell-permeable, stable dichlorosalicylaldehyde Schiff′s base that acts as a potent, selective inhibitor of Mia40/Erv1 redox-mediated import pathway (IC₅₀ = 700 nM, 900 nM, and 1.4 µM for ALR, Erv1, and Erv2, respectively). Significantly reduces the import of CX9 proteins, Erv1, Tim23, and ADP/ATP carrier (AAC). However, it does not affect mitochondrial membrane integrity as evidenced by the lack of aconitase, AAC, Tim54, Mia40, and cytochrome c release. Has no effect on protein disulfide isomerase, flavin adenine dinucleotide, and succinate dehydrogenase activities and does not disrupt mitochondrial net work or reduce viability of cells even at high concentrations (~100 µM in HeLa and HEK293 cells). Reported to specifically cause cytochrome c release, activate caspase-3, and induce apoptosis in human embryonic stem cells (~20 µM), but not in differentiated cells. Reversibly impairs cardiac development and reduces heart rate in zebra fish that is attributed to mitochondrial dysfunction.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Erv1

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Use only fresh DMSO for reconstitution.

Other Notes

Dabir, D.V., et al. 2013. Dev. Cell25, 81.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable