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420320

Sigma-Aldrich

KT5720

≥95% (HPLC), solid, protein kinase A inhibitor, Calbiochem®

Synonym(s):

KT5720, PKA Inhibitor VII

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About This Item

Empirical Formula (Hill Notation):
C32H31N3O5
CAS Number:
108068-98-0
Molecular Weight:
537.61
MDL number:
MFCD00132118
UNSPSC Code:
12352200
NACRES:
NA.77

product name

KT5720, A potent, specific, cell-permeable, reversible and ATP-competitive inhibitor of protein kinase A (Ki = 56 nM) that is prepared by a chemical modification of K-252a.

Quality Level

100

Assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

white

solubility

DMSO: 1 mg/mL

shipped in

ambient

storage temp.

−20°C

InChI

1S/C32H31N3O5/c1-3-4-5-10-15-39-30(37)32(38)16-23-34-21-13-8-6-11-18(21)25-26-20(17-33-29(26)36)24-19-12-7-9-14-22(19)35(28(24)27(25)34)31(32,2)40-23/h6-9,11-14,23,38H,3-5,10,15-17H2,1-2H3,(H,33,36)/t23-,31+,32+/m0/s1

InChI key

ZHEHVZXPFVXKEY-RUAOOFDTSA-N

General description

A potent, specific, cell-permeable, reversible and ATP-competitive inhibitor of protein kinase A (Ki = 56 nM) that is prepared by a chemical modification of K-252a. Does not significantly affect the activities of PKC, PKG, and MLCK. Inhibits axon branching in cultured neurons. A 2 mM (50 µg/47 µl) solution of KT 5720 (Cat. No. 420323) in DMSO is also available.
Prepared by a chemical modification of K-252a. A potent, specific, cell-permeable, reversible and ATP-competitive inhibitor of protein kinase A (Ki = 56 nM). Does not significantly affect the activity of PKC, PKG, or MLCK. Inhibits axon branching in cultured neurons.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
PKA
Product competes with ATP.
Reversible: yes
Target Ki: 56 nM for protein kinase A

Warning

Toxicity: Standard Handling (A)

Preparation Note

Further dilute with aqueous buffers just prior to use.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Simpson, C.S., and Morris, B.J. 1995. Neuroscience 68, 97.
Cabell, L., and Audesirk, G. 1993. Int. J. Dev. Neurosci.11, 357.
Gadbois, D.M., et al. 1992. Proc. Natl. Acad. Sci. USA89, 8626.
Kase, H., et al. 1987. Biochem. Biophys. Res. Commun.142, 436.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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