1.16493
Urease
(from jack beans) solution in 50% glycerol, 1000 U/ml for biochemistry EC 3.5.1.5
Synonym(s):
Urease enzyme
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.21
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form
liquid
pH
6.0-7.0 (20 °C in H2O)
density
1.10-1.30 g/cm3 at 20 °C
storage temp.
2-8°C
General description
Urease is a nickel-containing enzyme found in plants, bacteria, fungi, algae, and soil. It is a hexamer of a single chain of 840 amino acid residues. Urease is localized to mature seeds.
Biochem/physiol Actions
Ureases are metalloenzymes that catalyze the hydrolysis of urea to release ammonia (a nitrogen source for growth) and carbon dioxide. In plants, ureases are essential for the bioavailability/metabolism of nitrogen in nature and defense mechanisms. They exhibit internal peptides-mediated insecticidal action and fungicidal activity.
Analysis Note
Activity (Urea; pH 8.0; 25 °C): ≥ 1000 U/ml
Spec. activity (Urea; pH 8.0; 25 °C): about 500
Spec. activity (Urea; pH 8.0; 25 °C): about 500
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
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Urea in water and wastewater, enzymatic determination - Photometric determination using the indophenol blue method after cleavage of the urea
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