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07-363

Sigma-Aldrich

Anti-AMPK α2 Antibody

Upstate®, from rabbit

Synonym(s):

AMP-Activated Protein Kinase, AMPK

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_006252

UniProt accession no.

P54646

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... PRKAA2(5563)

Specificity

Recognizes AMP-Activated Protein Kinase alpha2 (AMPKα2).

Immunogen

peptide corresponding to amino acids 353-367 (C-DDSAMHIPPGLKPHP) of human AMP-Activated Protein Kinase alpha2 (AMPKalpha2) catalytic subunit

Application

Detect AMPK α2 using this Anti-AMPK α2 Antibody validated for use in WB.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Quality

routinely evaluated by immunoblot in a rat liver preparation

Target description

~63 kDa

Physical form

Format: Purified
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Protein A purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Rat liver preparation

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Miki Tadaishi et al.
American journal of physiology. Endocrinology and metabolism, 300(2), E341-E349 (2010-11-26)
There are three isoforms of peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α) mRNA, which promotes mitochondrial biogenesis in skeletal muscles. Compared with PGC-1α-a mRNA, PGC-1α-b or PGC-1α-c mRNA is transcribed by a different exon 1 of the PGC-1α gene. In this
TBC1D1 regulates insulin- and contraction-induced glucose transport in mouse skeletal muscle.
An, D; Toyoda, T; Taylor, EB; Yu, H; Fujii, N; Hirshman, MF; Goodyear, LJ
Diabetes null
Transcriptome and translational signaling following endurance exercise in trained skeletal muscle: impact of dietary protein.
Rowlands, DS; Thomson, JS; Timmons, BW; Raymond, F; Fuerholz, A; Mansourian, R; Zwahlen et al.
Physiological Genomics null
TBC 11251. IPI 1040.
Drugs in R&D, 2(1), 38-39 (1999-12-28)
Anja Böhm et al.
Diabetes, 65(10), 2849-2861 (2016-07-01)
A substantial number of people at risk of developing type 2 diabetes could not improve insulin sensitivity by physical training intervention. We studied the mechanisms of this impaired exercise response in 20 middle-aged individuals at high risk of developing type
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