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H-902

Supelco

α-Hydroxymidazolam solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

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About This Item

Empirical Formula (Hill Notation):
C18H13ClFN3O
CAS Number:
59468-90-5
Molecular Weight:
341.77
EC Number:
200-659-6
UNSPSC Code:
41116107
NACRES:
NA.24

grade

certified reference material

form

liquid

feature

Snap-N-Spike®/Snap-N-Shoot®

packaging

ampule of 1 mL

manufacturer/tradename

Cerilliant®

concentration

100 μg/mL in methanol

technique(s)

gas chromatography (GC): suitable
liquid chromatography (LC): suitable

application(s)

clinical testing

format

single component solution

storage temp.

−20°C

SMILES string

OCc1ncc2CN=C(c3ccccc3F)c4cc(Cl)ccc4-n12

InChI

1S/C18H13ClFN3O/c19-11-5-6-16-14(7-11)18(13-3-1-2-4-15(13)20)22-9-12-8-21-17(10-24)23(12)16/h1-8,24H,9-10H2

InChI key

QHSMEGADRFZVNE-UHFFFAOYSA-N

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General description

α-Hydroxymidazolam is an active, major metabolite in urine and blood of the benzodiazepine, midazolam. Midazolam is sold as Dormicum, Hypnovel®, and Versed as a sedative and treatment for insomnia and seizures. This Certified Spiking Solution® is suitable for use as starting material in calibrators or controls for a variety of LC/MS or GC/MS applications from forensic analysis and clinical toxicology to urine drug testing.

Application


  • α-Hydroxymidazolam solution in pharmacokinetic studies: The application of α-Hydroxymidazolam solution is crucial in pharmacokinetic research to study the metabolism of midazolam, particularly its phase I metabolic pathways, providing essential data on the drug′s biotransformation and clearance rates in humans (Bourget et al., 1996).

  • α-Hydroxymidazolam as a benzodiazepine metabolite standard: Utilized as a benzodiazepine metabolite standard, α-Hydroxymidazolam aids in the accurate measurement of midazolam and its metabolites in plasma using high-performance liquid chromatography, essential for ensuring dosing efficacy and safety in clinical settings (Lee and Charles, 1996).

Legal Information

CERILLIANT is a registered trademark of Merck KGaA, Darmstadt, Germany
CERTIFIED SPIKING SOLUTION is a registered trademark of Cerilliant Corporation
Hypnovel is a registered trademark of Hoffman-LaRoche & Co. AG
Snap-N-Shoot is a registered trademark of Cerilliant Corporation
Snap-N-Spike is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

H-921

α-Hydroxymidazolam-d4 solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

H-922

α-Hydroxymidazolam solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-908

Midazolam solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-918

Midazolam-d4 maleate, 100 μg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

H-156

4-Hydroxymidazolam-D4 solution, 100 μg/mL in acetonitrile, certified reference material, ampule of 1 mL, Cerilliant®

H-172

4-Hydroxymidazolam solution, 1 mg/mL (90:10 Acetonitrile:DMSO), certified reference material, ampule of 1 mL, Cerilliant®

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Target Organs

Eyes

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

49.5 °F - closed cup

Flash Point(C)

9.7 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Camilla Svanström et al.
Journal of pharmaceutical and biomedical analysis, 58, 71-77 (2011-10-14)
The metabolic conversion of midazolam (MDZ) to its main metabolite 1'-hydroxy-midazolam (1-OH-MDZ) can be used as a probe drug for cytochrome P450 3A (CYP3A) activity. A sensitive method for the simultaneous determination of MDZ and its metabolite 1-OH-MDZ in human
J Yang et al.
Clinical pharmacology and therapeutics, 91(3), 442-449 (2011-11-04)
The allosteric effect of fluconazole (effector) on the formation of 1'-hydroxymidazolam (1'-OH-MDZ) and 4-hydroxymidazolam (4-OH-MDZ) from midazolam (MDZ), a substrate of CYP3A4/5--members of the cytochrome P450 superfamily of enzymes--was examined in healthy volunteers. Following pretreatment with fluconazole, the ratio of
Zhe-Yi Hu et al.
Drug metabolism and disposition: the biological fate of chemicals, 38(5), 817-823 (2010-02-19)
Controversy exists concerning the sex-dependent differences in cytochrome P450 3A activity in humans. Meta-analysis of selected studies may address this question. Meta-analysis was performed on published or unpublished data in terms of sex-dependent differences in midazolam (MDZ) disposition in humans.
Ruut Kaartama et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(19), 1668-1676 (2011-05-03)
A sensitive and selective gas chromatographic mass spectrometric method for the determination of midazolam and its biologically active metabolite, 1-hydroxymidazolam, in rabbit plasma has been developed and validated. Sample preparation includes mixed-mode solid-phase extraction and derivatization with silylating reagents. Midazolam-d4
High-throughput analysis of in vitro cytochrome p450 inhibition samples using mass spectrometry coupled with an integrated liquid chromatography/autosampler system.
Ann Brown et al.
Rapid communications in mass spectrometry : RCM, 24(8), 1207-1210 (2010-03-20)
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