ZRB1036

Anti-PVR (CD155) Antibody, clone 4B3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): CD155, NECL-5, Nectin-like protein 5, Poliovirus receptor
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit (recombinant)
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4B3, recombinant monoclonal; monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 45.3 kDa; calculated mol wt ~65 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: P15151
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... PVR(5817)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 4B3 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human poliovirus receptor. It targets an epitope within 16 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human poliovirus receptor.

Application

Anti-PVR (CD155), clone 4B3 ZooMAb, Cat. No. ZRB1036, is a recombinant Rabbit monoclonal antibody that targets PVR (CD155/Poliovirus receptor) and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PVR (CD155) in HUVEC lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PVR (CD155) in A431 cells.

Affinity Binding Assay: A representative lot of this antibody bound PVR (CD155) peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected PVR (CD155) in U937 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Poliovirus receptor (UniProt: P15151; also known as Nectin-like protein 5, NECL-5, CD155) is encoded by the PVR (also known as PVS) gene (Gene ID: 5817) in human. CD155 is a single-pass type I, N-glycosylated, membrane protein that mediates NK cell adhesion and triggers NK cell effector functions. It is ubiquitously expressed in various tissues and many human tumors are reported to significantly upregulate its expression. It binds two different NK cell receptors: CD96 and CD226 and these interactions accumulate at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and the target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. CD155 serves as a receptor for poliovirus and may play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with dynein light chain Tctex-type 1 (DYNLT1). It is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 21-343), a transmembrane domain (aa 344-367), and a cytoplasmic domain (aa 368-417). It contains two Ig-like C2 type domains (aa 145-237 and 233-328), and an Ig-like V-type domain that is shown to be necessary and sufficient for virus binding and uptake. CD155 also has one the immunoreceptor tyrosine-based inhibitor motif (ITIM; aa 396-401) that when phosphorylated by Src kinases binds to the SH2 domain of several SH2-containing phosphatases. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tahara-Hanaoka, S., et al. (2004). Int. Immunol. 16(4); 533-538; Iguchi-Manaka, A., et al. (2016). PLoS One. 11(4); e0152982).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable