U9257
UbcH9 Conjugating Enzyme human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
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About This Item
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recombinant
expressed in E. coli
Quality Level
Assay
≥95% (SDS-PAGE)
form
buffered aqueous glycerol solution
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... UBE2E3(10477) , UBE2I(7329)
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General description
Manufactured for Sigma by Boston Biochem., Inc.
Biochem/physiol Actions
UbcH9 mediates the conjugation of SUMO-1, SUMO-2, and SUMO-3 to a diverse variety of proteins without the help of an E3 ligase. This E2 conjugating enzyme interacts directly with protein substrates that are modified by SUMOylation, and may help in substrate recognition. UbcH9 is specific for the conjugation of proteins modified by SUMOylation.
Physical form
Solution in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10 % glycerol, 1mM TCEP.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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FEBS letters, 417(3), 297-300 (1997-12-31)
Ubiquitin conjugating enzymes participate in the thioester cascade that leads to protein ubiquitination. Although Ubc9 is homologous to E2 ubiquitin conjugating enzymes we have shown that it is unable to form a thioester with ubiquitin, but can form a thioester
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