SRP0247

MAP3K14(NIK) Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Synonym(s): MAP3K14, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, NIK
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥70% (SDS-PAGE)
• form: aqueous solution
• mol wt: 88 kDa
• packaging: pkg of 10 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_003954
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... MAP3K14(9020)

Description

General description

Human MAP3K14 (mitogen-activated protein kinase kinase kinase 14 ; NIK ; GenBank Accession No. NM_003954), (amino acids 318-end) with N-terminal GST tag, MW = 88 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.