SAB4200862

Anti-Salmonella enterica typhimurium antibody produced in rabbit

IgG fraction of antiserum

• Synonym(s): Salmonella typhimurium (Loeffler) Castellani and Chalmers
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• form: liquid
• species reactivity: (Salmonella enterica typhimurium)
• packaging: pkg of 100 μL; pkg of 25 μL
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 1:2000-1:4000 using Salmonella enterica typhimurium LPS; indirect ELISA: 1:2000-1:4000 using using whole Salmonella
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Salmonella is a genus of Gram-negative, facultative anaerobe, motile bacillus composed of 2579 different serotypes. Salmonella is considered to be one of the most studied microorganisms as a worldwide foodborne pathogen and a major cause of gastrointestinal human infections.1 S. enterica is a subspecies of S. enterica, that includes 1531 serotypes, among them S. typhimurium and S. enteritidis, responsible for common human illness and thus focus of extensive research.1

Specificity

almonella enterica typhimurium antibody recognizes S. enterica typhimurium whole lysate of dead bacteria and S. enterica typhimurium Lipopolysaccharides (LPS), the antibody has no cross reactivity with whole bacterial extracts of E. coli K-12 and E. faecalis.

Application

The antibody may be used in various immunochemical techniques including Immunoblotting and ELISA. Detection of the S. typhimurium bands by Immunoblotting is specifically inhibited by the immunogen.

Biochem/physiol Actions

S. typhimurium can survive the acidic environment ofthe stomach through the activation of acid tolerance response (ATR), which maintains the intracellular pH
higher than the extracellular pH.6 Then, S. typhimurium penetrates the mucus barrier of the intestinal wall and adheres to the epithelium to initiate the infection, that may results in diarrhea, loss of electrolytes and local inflammation of the intestine.7-9
After S. typhimurium is being engulfed by enterocytes, it forms intracellular phagosomal compartments, termed Salmonella-Containing Vacuoles (SCV) where the bacterium is able to survive and multiply.2,9 S. typhimurium has various virulence factors, most of them are enoced by genes located in the Salmonella Pathogenicity Islands (SPI).10 S. typhimurium uses flagella for motility after ingestion11 and then type I fimbrae12 and adhesins to adhere to the epithelium. It expresses Type III Secretion System (T3SS) that enables the bacteria to inject into the host cells a variety of effector proteins such as, SipABCD, SptP, SpiC and SopB that alter cellular functions to promote bacterial invasion, survival and propagation.2,13,14 Moreover, S. typhimurium is able to form resistant biofilms15 that contribute to the establishment of chronic, ""carrier state"" infections. In addition, human specific S. typhiis used as a model for the animal study of typhoid fever.16

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1