SAB4200712

Anti-MAPK3 antibody, Mouse monoclonal

clone mapk3-4, purified from hybridoma cell culture

• Synonym(s): Anti-ERK1, Anti-ERT2 or Anti-p44-MAPK, Anti-p44-ERK1
• UNSPSC Code: 12352202
• NACRES: NA.44

Properties

• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: mapk3-4, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~43 kDa
• species reactivity: human, monkey
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 2-4 μg/mL using whole extract of human HepG2 cells; immunoprecipitation (IP): 5-10 μg/test using total extract of A431 cells
• isotype: IgG2a
• UniProt accession no.: P27361
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAPK3(5595)

Description

General description

Anti-MAPK3 antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the mapk3-4 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse. Mitogen-activated protein kinase 3 (MAPK3) is a serine/threonine protein kinase. It is an essential component of the MAP kinase signal transduction pathway.

Specificity

Monoclonal Anti-MAPK3 specifically recognizes human and monkey MAPK3. Monoclonal Anti-MAPK3 does not show cross reactivity with MAPK1 (ERK2)

Immunogen

synthetic peptide from the N-terminal region of human MAPK3, conjugated to KLH.

Application

Anti-MAPK3 antibody, Mouse monoclonal may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Mitogen-activated protein (MAP) kinase signal transduction pathway participates in the regulation of a large variety of processes, including cell adhesion, cell cycle progression, cell migration, cell survival, differentiation, metabolism, proliferation and transcription. Mitogen-activated protein kinase 3 (MAPK3) is phosphorylated on Thr-202 and Tyr-204 due to activation by upstream kinases, such as MAP2K1/MEK1 and MAP2K2/MEK2, which results in translocation into the nucleus where it, in turn, phosphorylates nuclear targets. MAPK3 is known as an important target in the diagnosis and treatment of several diseases including cancer, cardiac hypertrophy and influenza A infection.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable