Skip to Content
Merck
All Photos(3)

Key Documents

View All Documentation

S9626

Sigma-Aldrich

Sulfatase from Helix pomatia

Type H-1, sulfatase ≥10,000 units/g solid

Synonym(s):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

CAS Number:
9016-17-5
Enzyme Commission number:
3.1.6.1 (BRENDA, IUBMB)
EC Number:
232-772-1
MDL number:
MFCD00132399
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type H-1

Quality Level

200

form

powder

sulfatase activity

≥10,000 units/g solid

secondary activity

≥300 unit/mg solid β-glucuronidase (at pH 5.0)

mol wt

~85 kDa by gel filtration

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Application

Sulfatase from Helix pomatia has been used in:
  • sample extraction
  • sulfatase assays
  • enzymatic hydrolysis
  • extract preparation for glucosinolate analysis

Biochem/physiol Actions

Sulfatase from Helix pomatia facilitates the synthesis of thiohydroximates. It causes the hydrolysis of β-naphthyl sulfate much faster than a-naphthyl sulfate and possesses regiospecificity in the hydrolysis of ortho and para substituted phenyl sulfates.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

substrate

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Use of diabetic test kits to assess the recovery of glucosinolates during isolation of canola protein
Ser W Y, et al.
Food Sci. Technol., 41(5), 934-941 (2008)
Glucosinolate enhancement in cabbage induced by jasmonic acid application.
Fritz, V. A., Justen, V. L., Bode, A. M., Schuster, T., & Wang, M.
Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 45, 1188-1191 (2010)
Simultaneous analysis of several non-steroidal anti-inflammatory drugs in human urine by high-performance liquid chromatography with normal solid-phase extraction
Hirai T, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 692(2), 375-388 (1997)
Ivica Blažević et al.
Phytochemistry, 169, 112100-112100 (2019-11-28)
The glucosinolates (GSLs) is a well-defined group of plant metabolites characterized by having an S-β-d-glucopyrano unit anomerically connected to an O-sulfated (Z)-thiohydroximate function. After enzymatic hydrolysis, the sulfated aglucone can undergo rearrangement to an isothiocyanate, or form a nitrile or
A single monooxygenase, ese, is involved in the metabolism of the organochlorides endosulfan and endosulfate in an Arthrobacter sp.
Weir K M, et al.
Applied and Environmental Microbiology, 72(5), 3524-3530 (2006)
View All Related Papers

Protocols

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service