Yes, it can be used on formalin-fixed tissue, but one would need to amplify the gene of interest before bisulfite treatment
MOD50
Imprint® DNA Modification Kit
For bisulfite DNA conversion & purification
Synonym(s):
Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification
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1 KIT
8 310,00 kr
About This Item
UNSPSC Code:
41116158
EC Number:
231-548-0
NACRES:
NA.54
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This Item | |||
|---|---|---|---|
| usage sufficient for 50 reactions | usage - | usage - | usage - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 20-25°C | storage temp. −20°C | storage temp. - | storage temp. −20°C |
General description
The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
Application
Imprint® DNA Modification Kit has been used for bisulfite modification of DNA.
Features and Benefits
- Only 50 picograms of DNA or 20 cells are required
- Procedure takes less than 2 hours
- Greater than 99% conversion rate
- Extremely low degradation
- Option of convenient one-step protocol
- Consistent and reproducible Bisulfite Modification
- Can be used with genomic, endonuclease digested, and FFPE DNA
Preparation Note
All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit Components Also Available Separately
Product No.
Description
SDS
- T3566Clear-view™ Snap-Cap microtubes, size 1.5 mL, natural
signalword
Danger
supp_hazards
Storage Class
8B - Non-combustible corrosive hazardous materials
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
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David E Condon et al.
BMC bioinformatics, 19(1), 31-31 (2018-02-07)
Identification of differentially methylated regions (DMRs) is the initial step towards the study of DNA methylation-mediated gene regulation. Previous approaches to call DMRs suffer from false prediction, use extreme resources, and/or require library installation and input conversion. We developed a
Elena Ivanova et al.
Clinical epigenetics, 12(1), 64-64 (2020-05-13)
Preimplantation embryos experience profound resetting of epigenetic information inherited from the gametes. Genome-wide analysis at single-base resolution has shown similarities but also species differences between human and mouse preimplantation embryos in DNA methylation patterns and reprogramming. Here, we have extended
Priyadarshini Kachroo et al.
Epigenomics, 10(2), 133-147 (2018-01-16)
To determine whether methylation differences between mostly fatal TCF3-HLF and curable TCF3-PBX1 pediatric acute lymphoblastic leukemia subtypes can be associated with differential gene expression and remission. Five (extremely rare) TCF3-HLF versus five (very similar) TCF3-PBX1 patients were sampled before and
Matilde Y Follo et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(39), 16811-16816 (2009-10-07)
Lipid signaling pathways are involved in cell growth, differentiation, and apoptosis, and could have a role in the progression of myelodysplastic syndromes (MDS) into acute myeloid leukemia (AML). Indeed, recent studies showed that phosphoinositide-phospholipase (PI-PL)Cbeta1 mono-allelic deletion correlates with a
Nelly Olova et al.
Genome biology, 19(1), 33-33 (2018-03-17)
Whole-genome bisulfite sequencing (WGBS) is becoming an increasingly accessible technique, used widely for both fundamental and disease-oriented research. Library preparation methods benefit from a variety of available kits, polymerases and bisulfite conversion protocols. Although some steps in the procedure, such
Articles
Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.
Protocols
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Related Content
The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.
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Can Product MOD50, Imprint® DNA Modification Kit, be used on formalin-fixed tissue ?
1 answer-
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How many reactions can be run with Product MOD50, Imprint® DNA Modification Kit?
1 answer-
This kit can be used for 50 reactions.
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Can I use Product MOD50, Imprint® DNA Modification Kit with DNA templates that contain high GC content or secondary structures?
1 answer-
Yes, but it will be necessary to increase the bisulfite reaction time to 150 to 180 min.
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What is the minimum amount of DNA that can be used with Product MOD50, Imprint® DNA Modification Kit?
1 answer-
DNA amounts ranging from 0.1 ng to 1 mg have been used successfully. For optimal modification, the DNA quantity should be in the range of 50-200 ng.
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Can you store the DNA modified using Product MOD50, Imprint® DNA Modification Kit?
1 answer-
The modified DNA generated with this kit can be stored for 2 months at -20 °C, 6 months at -80 °C.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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