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FLE250

Firefly Lantern Extract

for ATP determination

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Synonym(s):

FLE

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Size/SKUAvailabilityPrice
1 vial
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1 440,00 kr

About This Item

NACRES:
NA.81
UNSPSC Code:
12352202
Form:
solution

1 440,00 kr

Save Up To 15 %

Please contact Customer Service for Availability
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Quality Segment

form

solution

packaging

pkg of 250 mg dried lanterns

technique(s)

protein detection: suitable, toxicology assay: suitable

storage temp.

−20°C

General description

The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.

Application

Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells.
Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.

Biochem/physiol Actions

Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg2+ to yield a light-emitter oxyluciferin.

Preparation Note

After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO4.

Other Notes

Crude luciferin and luciferase

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This Item
F6303L9420SCT154
Firefly Lantern Extract for ATP determination

Sigma-Aldrich

FLE250

Firefly Lantern Extract

Firefly Lantern Extract pkg of 100 mg

Sigma-Aldrich

F6303

Firefly Lantern Extract

Luciferase from Photinus pyralis (firefly) recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein

Sigma-Aldrich

L9420

Luciferase from Photinus pyralis (firefly)

Firefly Luciferase Assay Flash-type luciferase assay designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity.

Sigma-Aldrich

SCT154

Firefly Luciferase Assay

description

for ATP determination

description

pkg of 100 mg

description

recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein

description

Flash-type luciferase assay designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity.

technique(s)

toxicology assay: suitable

technique(s)

toxicology assay: suitable

technique(s)

-

technique(s)

cell based assay: suitable

form

solution

form

solid

form

buffered aqueous solution

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-65 to -96°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

packaging

pkg of 250 mg dried lanterns

packaging

pkg of 100 mg

packaging

-

packaging

-

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    032K7460
    041K74151
    SLBG6458V
    SLBT4266
    101M7402

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