F7053
Anti-Vinculin−FITC antibody, Mouse monoclonal
clone hVIN-1, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Vinculin antibody produced in mouse
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200 μL
5 070,00 kr
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200 μL
5 070,00 kr
About This Item
conjugate:
FITC conjugate
application:
IF (d)
clone:
hVIN-1, monoclonal
species reactivity:
frog, chicken, mouse, canine, human, bovine, rat, turkey
citations:
18
technique(s):
direct immunofluorescence: 1:50 using methanol/acetone fixed cultured bovine MDBK cells
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biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
hVIN-1, monoclonal
form
buffered aqueous solution
mol wt
antigen 116 kDa
species reactivity
frog, chicken, mouse, canine, human, bovine, rat, turkey
storage condition
protect from light
technique(s)
direct immunofluorescence: 1:50 using methanol/acetone fixed cultured bovine MDBK cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)
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Specificity
Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.
Immunogen
purified human vinculin from uterus.
Application
Monoclonal Anti-Vinculin-FITC antibody is suitable for immunofluorescence staining of fibroblasts cells to study the effects of nanofiber spatial organization on cell behaviors. It is also suitable for direct immunofluorescence at a dilution of 1:50 using methanol/acetone fixed cultured bovine MDBK cells.
Biochem/physiol Actions
Vinculin is a cytoskeletal protein with molecular mass of ~116 kDa. Vinculin is closely associated with adhesive membrane areas, such as focal contacts, podosomes, or intercellular junctions of the zonula adherens type. It acts as a mediator in interactions of integrins and the actin cytoskeleton. It forms a bridge between integrins, receptors for ECM molecules on the cell surface and the F-actin cytoskeleton. It is associated with talin and α-actinin which directly connects with integrins. It has ability to bind α-catenin as well as the vasodilator stimulated phosphoprotein (VASP) in focal contact assembly.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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E E Weiss et al.
The Journal of cell biology, 141(3), 755-764 (1998-06-13)
In epithelial cells, alpha-, beta-, and gamma-catenin are involved in linking the peripheral microfilament belt to the transmembrane protein E-cadherin. alpha-Catenin exhibits sequence homologies over three regions to vinculin, another adherens junction protein. While vinculin is found in cell-matrix and
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