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F2055

Sigma-Aldrich

Anti-hFBXW7 antibody , Mouse monoclonal

clone FB407, purified from hybridoma cell culture

Synonym(s):

Anti-ARCHIPELAGO (Drosophila), Anti-Cdc4 (S. serevisia), Anti-F-box and WD40 domain protein 7, Anti-FBW7, Anti-SEL-10 (C. elegance)

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About This Item

MDL number:
MFCD09265423
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

FB407, monoclonal

form

buffered aqueous solution

mol wt

antigen ~60 kDa

species reactivity

rat, human, mouse

concentration

~2 mg/mL

technique(s)

immunocytochemistry: 10-20 μg/mL using human A431 or HeLa cells
indirect ELISA: suitable
western blot: 5-10 μg/mL using human A431 cell extracts

isotype

IgG2a

UniProt accession no.

Q969H0

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FBXW7(55294)
mouse ... Fbxw7(50754)

General description

FBXW7 is a member of F-box protein family, which acts as a substrate recognition component of the ubiquitin ligase complex SCF (Skp-Cullin-F-box). The proteins have a bipartite structure. The shared F-box motif links F-box protein to Skp1 and the core complex, whereas divergent protein-protein interaction motifs selectively bind their cognate substrates. There are three FBXW7 isoforms α, β and γ that share 10 out of 11 exons but they differ in their subcellular localization as well as in substrate recognition activity.
Monoclonal Anti-hFBXW7 (mouse IgG2a isotype) is derived from the hybridoma FB407 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino-acids of human hFBXW7 isoforms α β and γ. FBXW7 is located on human chromosome 4q31.

Specificity

The antibody recognizes human, mouse, and rat hFBXW7.

Immunogen

synthetic peptide corresponding to amino-acids 238-250 of human hFBXW7 isoforms α, β, and γ.

Application

Anti-hFBXW7 antibody, Mouse monoclonal has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry

Biochem/physiol Actions

F-box proteins is associated with various signalling pathways, such as nutrient sensing in yeast,conserved developmental pathways in plants and animals. These proteins mediate recognition of phosphorylated targets including Cyclin E, Myc, c-Jun, and Notch, leading to their ubiquitination and degradation. Furthermore, it was found mutated in breast, endometrial, ovarian, and colon cancer, and finally results in genomic instability. Similar to the inactivation mode of other known tumor suppressors, the SV40 large T antigen binds FBXW7 without detectible effects on its stability, and thus acts as an inhibitor of FBXW7 activity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The Fbxw7/hCdc4 tumor suppressor in human cancer
Tan YM, et al.
Cancer letters, 271(1), 1-12 (2008)
Nicole E Willmarth et al.
Breast cancer research : BCR, 6(5), R531-R539 (2004-08-21)
Cyclin E, a G1 cyclin essential for G1-S phase transition, is known to have a profound effect on tumorigenesis. Elevated levels of cyclin E have been associated with breast cancer, and chromosomal instability observed in breast cancer is suggested to
Harith Rajagopalan et al.
Nature, 428(6978), 77-81 (2004-03-06)
Aneuploidy, an abnormal chromosome number, has been recognized as a hallmark of human cancer for nearly a century; however, the mechanisms responsible for this abnormality have remained elusive. Here we report the identification of mutations in hCDC4 (also known as
Markus Welcker et al.
The Journal of biological chemistry, 280(9), 7654-7658 (2004-12-22)
Cell transformation by simian virus 40 (SV40) results mostly from the highly oncogenic activities of the large T antigen (LT), which corrupts the cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. The most
Masayoshi Yada et al.
The EMBO journal, 23(10), 2116-2125 (2004-04-23)
The F-box protein Skp2 mediates c-Myc ubiquitylation by binding to the MB2 domain. However, the turnover of c-Myc is largely dependent on phosphorylation of threonine-58 and serine-62 in MB1, residues that are often mutated in cancer. We now show that
View All Related Papers

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