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About This Item
Empirical Formula (Hill Notation):
C192H292N50O58S5
CAS Number:
Molecular Weight:
4389.00
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32
Recommended Products
Quality Level
Assay
≥97% (HPLC)
UniProt accession no.
storage temp.
−20°C
Gene Information
rat ... Edn1(24323)
Amino Acid Sequence
Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-Val-Asn-Thr-Pro-Glu-Arg-Val-Val-Pro-Tyr-Gly-Leu-Gly-Ser-Pro-Ser-Arg-Ser [Disulfide Bridges: 1-15; 3-11]
General description
Big endothelin-1 is a 39 amino acid peptide, which is synthesized and secreted by vascular smooth muscle and endothelium cells. It is produced as an inactive enzyme, and is cleaved and activated by serine and aspartic proteases and metalloproteinases. Activated form is called ET-1, which is a potent vasoconstrictor. ET-1 is also produced by the action of endothelin converting enzyme (ECE) on big-ET-1. In guinea pigs, big-ET-1 causes the release of prostanoids and thromboxanes.
Application
Big Endothelin 39 rat has been used for charting of a standard curve in the study of the role of ET-1 in ventricular dilatation.
Biochem/physiol Actions
Precursor of Endothelin-1; vasoconstrictor activity appears to be due to its conversion to endothelin 1 by endothelin converting enzyme in the tissue.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 1 Inhalation - Acute Tox. 1 Oral
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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C Fernandez-Patron et al.
Circulation research, 85(10), 906-911 (1999-11-13)
Matrix metalloproteinase-2 (MMP-2, gelatinase A) and its tissue inhibitor (TIMP-2) are mainly known for their roles in the (patho)physiological remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. A mechanism of action of MMP-2 is
E G McMahon et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(3), 703-707 (1991-02-01)
In porcine aortic endothelial cells, the 21-amino acid peptide endothelin-1 (ET-1) is formed from a 39-amino acid intermediate called "big endothelin-1" (big ET-1) by a putative ET-converting enzyme (ECE) that cleaves the 39-mer at the bond between Trp-21 and Val-22.
S Lehoux et al.
British journal of pharmacology, 107(4), 996-1000 (1992-12-01)
1. Changes in vascular permeability following intravenous injections of human big-endothelin-1 (big-ET-1) and endothelin-1 (ET-1) were measured by extravasation of Evans blue dye (EB, 20 mg kg-1) in selected tissues. 2. A low dose of big-ET-1 (40 pmol kg-1) failed
D Fraccarollo et al.
Circulation, 96(11), 3963-3973 (1997-12-24)
Left ventricular dilatation after myocardial infarction is associated with impaired ventricular function and heart failure and has important implications for survival. The purpose of this study was to investigate the role of endothelin-1 (ET-1) in ventricular dilatation and the effects
T Sakurai et al.
Biochemical and biophysical research communications, 175(1), 44-47 (1991-02-28)
We report the cloning of a full-length cDNA encoding rat preproendothelin-1 (preproET-1). The predicted rat preproET-1 consists of 202 amino acid residues and highly similar to human, porcine and bovine preproET-1, respectively. The deduced 21-residue sequence of mature rat ET-1
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