RAW 264.7 Cell Line murine
91062702, mouse blood, Macrophage
Synonym(s):
RAW264 7 Cells, RAW264.7 Cells
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About This Item
UNSPSC Code:
41106514
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product name
RAW 264.7 Cell Line murine, Macrophage from blood, 91062702
biological source
mouse blood
growth mode
Semi-adherent
karyotype
Not specified
morphology
Macrophage
products
Lysozyme
receptors
Immunoglobulin, complement
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse monocyte macrophage
Cell Line Description
Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.
Application
Metabolic studies
RAW 264.7 cell line has been used to study the anti-inflammatory effects of pristimerin. It has also been used to test the internalization and intracellular survival of Listeria monocytogenes.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2; 5% CO2; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.
Other Notes
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Certificates of Analysis (COA)
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