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product name
IA-Xs SBR, 88090801
biological source
rat intestine
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Holtzmann rat small bowel adenocarcinoma
Cell Line Description
Derived from X-irradiation induced small bowel adenocarcinoma in adult male Holtzmann rat. Sensitive to lysis by peripheral blood lymphoid cells taken from F344 rats with colon and pancreatic cancers induced by 1, 2 Dimethylhydrazine or 7, 12 DMBT.
Application
Tumourigenicity studies
Culture Medium
Ham′s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
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