BW5147.G.1.4.OUA/R.1
85082302, mouse blood, Lymphoblast
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About This Item
UNSPSC Code:
41106514
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Product Name
BW5147.G.1.4.OUA/R.1, 85082302
biological source
mouse blood
growth mode
Suspension
morphology
Lymphoblast
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
General description
For complete product information, please see BW5147.G.1.4.OUA/R.1
Cell Line Origin
Mouse AKR/J T cell lymphoma
Cell Line Description
This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100μM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.
Application
Fusion partner
Culture Medium
SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 1-6x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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