C8897
Collagen from rat tail
Bornstein and Traub Type I (Sigma Type VII), powder
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About This Item
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biological source
rat tail
form
powder
technique(s)
cell culture | mammalian: suitable
solubility
aqueous acid: soluble
UniProt accession no.
storage temp.
2-8°C
Gene Information
rat ... Col1a1(29393)
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Application
This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.
Biochem/physiol Actions
Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues.
Components
All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.
Preparation Note
This product was prepared by a modification of the extraction method of Bornstein, M.B., Lab. Invest., 7, 134 (1958). It is an acid soluble collagen. It dissolves in water with acetic acid added to pH 3.0 at 5 mg/mL, yielding an opalescent, colorless solution.
Other Notes
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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