This is a powdered product. The potency in solution is dependent upon the concentration at which it is prepared. The minimum potency specification for this product is 240 µg/mg. The lot specific value will be reported on the Certificate of Analysis. Please see the link below to review a sample Certificate:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A9528-5G0000247193.pdf
A9528
Amphotericin B solubilized
powder, suitable for cell culture, BioXtra
Synonym(s):
Amphotericin B from Streptomyces sp., Fungizone
Sign Into View Organizational & Contract Pricing
Select a Size
50 MG
876,00 kr
100 MG
1 530,00 kr
500 MG
6 950,00 kr
1 G
13 040,00 kr
5 G
47 470,00 kr
All Photos(6)
Select a Size
Change View
50 MG
876,00 kr
100 MG
1 530,00 kr
500 MG
6 950,00 kr
1 G
13 040,00 kr
5 G
47 470,00 kr
About This Item
Empirical Formula (Hill Notation):
C47H73NO17
CAS Number:
Molecular Weight:
924.08
Beilstein:
78342
MDL number:
UNSPSC Code:
12352207
eCl@ss:
34010400
PubChem Substance ID:
NACRES:
NA.76
Recommended Products
Product Name
Amphotericin B solubilized, powder, γ-irradiated, BioXtra, suitable for cell culture
Quality Level
sterility
γ-irradiated
product line
BioXtra
form
powder
technique(s)
cell culture | mammalian: suitable
antibiotic activity spectrum
fungi
yeast
Mode of action
cell membrane | interferes
storage temp.
2-8°C
SMILES string
C[C@H]1O[C@@H](O[C@@H]2C[C@@H]3O[C@](O)(C[C@@H](O)C[C@@H](O)[C@H](O)CC[C@@H](O)C[C@@H](O)CC(=O)O[C@@H](C)[C@H](C)[C@H](O)[C@@H](C)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\2)C[C@H](O)[C@H]3C(O)=O)[C@@H](O)[C@@H](N)[C@@H]1O
InChI
1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1
InChI key
APKFDSVGJQXUKY-INPOYWNPSA-N
Looking for similar products? Visit Product Comparison Guide
General description
Chemical structure: polyene
Application
Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.
Biochem/physiol Actions
Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.
Packaging
Solubilized Amphotericin B is packaged in amber glass bottle, with the contents inside an inserted fused cone.
Components
This product is a colloidal suspension of Amphotericin B, using deoxycholate as the solubilizing agent. The formulation is ~45% Amphotericin B and 35% sodium deoxycholate in sodium phosphate and sodium chloride.
Caution
Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.
Preparation Note
Amphotericin B is soluble at 19.6-20.4 mg/ml in water, at natural pH without heating, giving a yellowish clear to slightly hazy solution.
Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize.
It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL).
Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize.
It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL).
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture. Keep in a dry place.
The actual solid weight of the packaged product is calculated according to the UV purity.
Kit Components Only
Product No.
Description
- Amphotericin B ~ 45 %
- Sodium deoxycholate ~ 35 %
- Sodium phosphate balance
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
STOT RE 1
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
ORAI1 and ORAI2 modulate murine neutrophil calcium signaling, cellular activation, and host defense.
Derayvia Grimes et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(39), 24403-24414 (2020-09-16)
Calcium signals are initiated in immune cells by the process of store-operated calcium entry (SOCE), where receptor activation triggers transient calcium release from the endoplasmic reticulum, followed by opening of plasma-membrane calcium-release activated calcium (CRAC) channels. ORAI1, ORAI2, and ORAI3
Etienne Loire et al.
Heliyon, 6(10), e05146-e05146 (2020-10-22)
Contagious caprine pleuropneumonia is an infectious and contagious disease affecting goats and wildlife ruminants, mostly in Africa and Asia. It is caused by a mycoplasma, Mycoplasma capricolum susbp. capripneumoniae, which is very fastidious. This may be the reason why there
Devin Tran et al.
Cornea, 38(7), 901-904 (2019-04-19)
Fungal infections in lamellar keratoplasty are a growing concern. Optisol-GS does not contain an antifungal agent and supplementation with 0.255 μg/mL Amphotericin B (AmpB) has been considered. This study tested the ability of 0.255 μg/mL AmpB in Optisol-GS to eliminate
Yuki Yamamoto et al.
Reproduction, fertility, and development, 28(6), 673-681 (2014-11-06)
Endothelin (EDN) is a possible regulating factor of oviductal motility, which is important for the transport of gametes and embryo. To clarify the factors that control the secretion of EDN in the bovine oviduct, the expression of EDNs, EDN-converting enzymes
Nadeeka S Udawatte et al.
Frontiers in pharmacology, 11, 511-511 (2020-05-12)
Candida albicans is the major fungal species associated with superficial mucosal infections such as oral candidiasis as well as systemic mycoses with high morbidity and mortality. On top of the rising drug resistance, currently available antifungal agents have significant adverse
Articles
Prevent fungal, yeast, and mold contamination in cell cultures. Discover the best antifungal agent for your cultures with the extensive Sigma® antifungal collection.
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
-
what is the potency of Amphotericin B solubilized solution?
1 answer-
Helpful?
-
-
How much DSMO do I dissolve the 100mg powder in to achieve a stock concentration of 2.5mg/mL?
1 answer-
Amphotericin B is soluble in DMSO at 30-40 mg/mL. Add 100 mg powder to 40 mL DMSO in order to achieve a 2.5 mg/mL concentration.
Helpful?
-
-
What is the shelf life of Product A9528, Amphotericin B solubilized?
1 answer-
Product No. A9528 has a recommended retest date of 5 years from the initial QC release date. This date can be found on the lot-specific Certificate of Analysis.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What is the amount of Amphotericin B in Product No. A9528?
1 answer-
The package size is the amount of Amphotericin B present in the vial. If weighing the product, utilize the potency value on the lot specific Certificate of Analysis as a correction factor.
Helpful?
-
-
What concentration should Product A9528, Amphotericin B solubilized, be used at?
1 answer-
We recommend to use Product No. A9528 at 5.6 mg solid per Liter. Cell culture tested amphotericin B solubilized preparation is approximately 45% amphotericin B, 35% sodium deoxycholate, with the balance being sodium phosphate. The amphotericin B is at least 80% amphotericin B and 3-5% amphotericin A by HPLC. The exact amount of amphotericin B in the product can be found on the lot specific Certificate of Analysis under Potency.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
