A1293

Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Goat Anti-Mouse IgG (Fab specific)–AP
• MDL number: MFCD00162620
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: goat
• recombinant: expressed in goat
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: mouse
• should not react with: human
• technique(s): direct ELISA: 1:40,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50; western blot (chemiluminescent): 1:30,000
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice .

Specificity

Binds all mouse Igs. Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fab fragment. The product does not react with mouse IgG, Fc fragment, or human IgG.

Immunogen

Purified mouse IgG Fab fragment

Application

Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm.

Anti-Mouse IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat has been used in:

• enzyme-linked immunosorbent assay (ELISA){47
• immunohistochemistry
• western blot

Biochem/physiol Actions

Immunoglobulin G (IgG) helps in opsonization and antibody dependent cell mediated cytotoxicity. It mainly helps in immune defense.

Other Notes

Antibody adsorbed with human IgG.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide

Preparation Note

Adsorbed to reduce background staining with human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable