67351
Atto 620 NHS ester
BioReagent, suitable for fluorescence, ≥80% (coupling to amines)
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About This Item
Recommended Products
product line
BioReagent
Assay
≥80% (coupling to amines)
manufacturer/tradename
ATTO-TEC GmbH
transmittance
254 nm, >90%
620 nm, >90%
fluorescence
λex 620 nm; λem 641 nm in 0.1 M phosphate pH 7.0
λ
in ethanol (with 0.1% trifluoroacetic acid)
suitability
suitable for fluorescence
storage temp.
−20°C
Application
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 620 NHS ester Atto 540 Q NHS ester is used to label molecules such as proteins via conjugation to primary amine groups.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Simone Nicolardi et al.
Analytical chemistry, 84(5), 2512-2520 (2012-02-11)
Fluorescent protein labeling has been an indispensable tool in many applications of biochemical, biophysical, and cell biological research. Although detailed information about the labeling stoichiometry and exact location of the label is often not necessary, for other purposes, this information
Maria Strianese et al.
Journal of inorganic biochemistry, 104(6), 619-624 (2010-03-23)
In this paper we explore the use of fluorescently labeled cytochrome c peroxidase (CcP) from baker's yeast for monitoring nitric oxide (NO) down to the sub-micromolar level, by means of a FRET (Förster Resonance Energy Transfer) mechanism. The binding affinity
Maria Strianese et al.
Inorganic chemistry, 51(21), 11220-11222 (2012-10-18)
Fluorescently labeled cobalt peptide deformylase (Co-PDF) can be efficiently used as a fluorescence-resonance-energy-transfer-based sensing device for hydrogen sulfide (H(2)S). The proof of concept of our sensor system is substantiated by spectroscopic, structural, and theoretical results. Monohydrogen sulfide coordination to Co-PDF
Peter P T Surawski et al.
Molecular bioSystems, 4(7), 774-778 (2008-06-20)
The concept of optically encoding particles for solid phase organic synthesis has existed in the literature for several years. However, there remains a significant challenge to producing particles that are capable of withstanding harsh solvents and reagents whilst maintaining the
Darby Kozak et al.
Langmuir : the ACS journal of surfaces and colloids, 24(4), 1204-1211 (2007-12-11)
This study presents the use of flow cytometry as a high-throughput quantifiable technique to study multicomponent adsorption interactions between proteins and surfaces. Flow cytometry offers the advantage of high-throughput analysis of multiple parameters on a very small sampling scale. This
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