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40809

Sigma-Aldrich

Purine nucleoside phosphorylase

recombinant, expressed in E. coli, ≥120 U/mL

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About This Item

CAS Number:
9030-21-1
Enzyme Commission number:
2.4.2.1 (BRENDA, IUBMB)
MDL number:
MFCD00131739
UNSPSC Code:
12352204

recombinant

expressed in E. coli

form

buffered aqueous solution (100 mM phosphate buffer with 1 mM MgCl2)

specific activity

≥120 U/mL

storage temp.

−20°C

InChI

1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1

InChI key

MRWXACSTFXYYMV-FDDDBJFASA-N

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Application

Purine nucleoside phosphorylase is used to study nucleotide salvage pathways and purine metabolism. It is used to study purine nucleoside phosphorylase deficiency and responsible PNP genetic mutations.

Biochem/physiol Actions

Purine nucleoside phosphorylase is an enzyme involved in purine metabolism. PNP metabolizes adenosine into adenine, inosine into hypoxanthine, and guanosine into guanine. Mutations in the PNP gene are responsible for purine nucleoside phosphorylase deficiency.

Unit Definition

1 U will cause the phosphorolysis 1 μmol of inosine to hypoxanthine and ribose 1-phosphate per minute at pH 7.4 at 25°C

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Purine nucleoside phosphorylase deficiency with a novel PNP gene mutation: a first case report from India
Manisha Rajan Madkaikar, et al.
BMJ Case Reports, DOI:10-DOI:10 (2011)
Fernanda Canduri et al.
Biochemical and biophysical research communications, 313(4), 907-914 (2004-01-07)
Human purine nucleoside phosphorylase (PNP) is a ubiquitous enzyme which plays a key role in the purine salvage pathway, and PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effect on B-cell function. PNP
Anke Konrad et al.
Gene, 510(2), 154-161 (2012-09-13)
Nucleoside phosphorylases are essential for the salvage and catabolism of nucleotides in bacteria and other organisms, and members of this enzyme superfamily have been of interest for the development of antimicrobial and cancer therapies. The nucleotide phosphorylase superfamily 1 encompasses
Candida Deves et al.
Bone, 52(1), 167-175 (2012-10-03)
Purine nucleoside phosphorylase (PNP) is a purine-metabolizing enzyme that catalyzes the reversible phosphorolysis of 6-oxypurine (deoxy)nucleosides to their respective bases and (deoxy)ribose-1-phosphate. It is a key enzyme in the purine salvage pathway of mammalian cells. The present investigation sought to
Eric J Sorscher et al.
Cancer chemotherapy and pharmacology, 70(2), 321-329 (2012-07-05)
Systemically administered fludarabine phosphate (F-araAMP) slows growth of human tumor xenografts that express Escherichia coli purine nucleoside phosphorylase (PNP). However, this treatment has been limited by the amount of F-araAMP that can be administered in vivo. The current study was
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