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35845

Sigma-Aldrich

DCFHDA

BioReagent, suitable for fluorescence, ≥95% (HPLC)

Synonym(s):

2′,7′-Dichlorofluorescein diacetate, Diacetyldichlorofluorescein

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5 G
4 600,00 kr

4 600,00 kr

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5 G
4 600,00 kr

About This Item

Empirical Formula (Hill Notation):
C24H14Cl2O7
CAS Number:
2044-85-1
Molecular Weight:
485.27
Beilstein:
68488
MDL number:
MFCD00037501
UNSPSC Code:
12352108
PubChem Substance ID:
57649438
NACRES:
NA.32

4 600,00 kr

Please contact Customer Service for Availability
Request a Bulk Order

product line

BioReagent

Quality Level

100

Assay

≥95% (HPLC)

mp

232-234 °C (lit.)

solubility

DMSO: soluble

fluorescence

λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3

InChI key

VQVUBYASAICPFU-UHFFFAOYSA-N

General description

2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.[1]

Application

2′,7′-Dichlorofluorescein diacetate is predominantly used to detect reactive oxygen (ROS) intermediates and quantify the overall oxidative stress in cells.[1]

Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells.[2]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL7891
BCCM2610
BCCL2220
BCCK3188
BCCJ3620

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Laura Sartori Assunção et al.
Biochimica et biophysica acta. General subjects, 1863(9), 1332-1342 (2019-06-07)
In a previous study, we described a series of 28 aryl- and alkyl-substituted isothiouronium salts with antitumor activity and selectivity toward a leukemia cell line. Among the synthesized compounds, methyl (Z)-2-(isothioureidomethyl)-2-pentenoate hydrobromide (IS-MF08) showed conspicuous activity. In the present study
Makoto Ohtake et al.
Scientific reports, 7, 42783-42783 (2017-02-22)
We previously reported that μ-oxo N,N'-bis(salicylidene)ethylenediamine iron [Fe(Salen)], a magnetic organic compound, has direct anti-tumor activity, and generates heat in an alternating magnetic field (AMF). We showed that Fe(Salen) nanoparticles are useful for combined hyperthermia-chemotherapy of tongue cancer. Here, we
Zhen-Wei Zou et al.
Redox biology, 16, 226-236 (2018-03-12)
Thyroid cancer is the most common endocrine carcinoma with increasing incidence worldwide and anaplastic subtypes are frequently associated with cancer related death. Radioresistance of thyroid cancer often leads to therapy failure and cancer-related death. In this study, we found that
Somayeh Keshtkar et al.
Scientific reports, 9(1), 11701-11701 (2019-08-14)
Islets transplantation, as a treatment of type 1 diabetes, faces challenges, including the loss of islets in the process of isolation and pre-transplantation due to cellular stresses-induced apoptosis. Accordingly, the optimization of culture plays a decisive role in the transplantation
Pauliina Saurus et al.
Scientific reports, 7(1), 10731-10731 (2017-09-08)
Lack of CD2-associated protein (CD2AP) in mice increases podocyte apoptosis and leads to glomerulosclerosis and renal failure. We showed previously that SHIP2, a negative regulator of the PI3K/AKT signalling pathway, interacts with CD2AP. Here, we found that the expression level
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Questions

1–8 of 8 Questions  

  1. Can I get the protocol? Thank you

    1 answer

    1. A protocol is not available for this compound.

      Please see the link below to review a publication that may be helpful.
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712457/

      Helpful?

  2. What is the shelf life of this product, as a powder, and once dissolved?

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Stock solutions of this item in organic solvents are stable for at least 6 months if stored at -20°C.

      Helpful?

  3. What are the optical properties of the hydrolysis product of 2´,7´-Dichlorofluorescein diacetate, Product 35845?

    1 answer

    1. Excitation maximum is at 504 nm; emission maximum is at 529 nm (in 0.1 M Tris pH 8.0 following treatment with an esterase).

      Helpful?

  4. What are the recommended storage conditions for 2´\,7´-Dichlorofluorescein diacetate, Product 35845?

    1 answer

    1. Product no. 35845 should be stored in a refrigerator or coldroom at 2-8°C.

      Helpful?

  5. Can Product 35845, 2´,7´-Dichlorofluorescein diacetate be used for ROS (Reactive Oxygen Species) detection?

    1 answer

    1. Yes, Product 35845, 2´,7´-Dichlorofluorescein diacetate can be used for ROS detection by FACS analysis.

      Helpful?

  6. In what solvents is 2´,7´-´Dichlorofluorescein diacetate, Product 35845, soluble?

    1 answer

    1. It is soluble in DMF, ethanol (2 mg/mL), DMSO (10 mg/mL).

      Helpful?

  7. What is required to get 2´,7´-Dichlorofluorescein diacetate, Product 35845, to fluoresce?

    1 answer

    1. 2',7'-dichlorofluorescein diacetate fluoresces only after cleavage of the acetyl groups.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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