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00289

Sigma-Aldrich

Anti-Rabbit IgG−Abberior® STAR 512 antibody produced in goat

for STED application

Synonym(s):

Abberior® STAR 512-Anti-Rabbit IgG antibody produced in goat

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

goat

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rabbit

concentration

~1 mg/mL

fluorescence

λex 511 nm; λem 530 nm in PBS, pH 7.4

storage temp.

−20°C

General description

Abberior STAR 512 was developed for STED and confocal microscopy in the green spectral region. Abberior STAR 512 is highly photostable and bright green fluorescent dye. The dye works extremely well with Abberior Instruments Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with excitation lasers at 488 nm or 514 nm. Abberior STAR 512 can substitute dyes like Oregon Green 514, Atto™ 514 or Alexa Fluor™ 514. For STED microscopy, Abberior STAR 512 can be most efficiently used with STED Laser wavelengths between 590 - 610 nm. Best results are obtained with freshly prepared samples.

Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 511 (PBS pH 7.4), 512 (H2O), 517 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 85 000 (PBS pH 7.4), 92 000 (H2O), 95 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,24 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,07 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 530 (PBS pH 7.4), 530 (H2O), 533 (MeOH)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,82 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (PBS pH 7.4)

Suitability

Designed and tested for fluorescent super-resolution microscopy

Analysis Note

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Other Notes

Find more infomation here Abberior®

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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S. M. Polyakova, et al.,
European Journal of Organic Chemistry, 30, 5162-5177 (2009)
Gyuzel Yu Mitronova et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 16(15), 4477-4488 (2010-03-24)
New photostable rhodamine dyes represented by the compounds 1 a-r and 3-5 are proposed as efficient fluorescent markers with unique combination of structural features. Unlike rhodamines with monoalkylated nitrogen atoms, N',N-bis(2,2,2-trifluoroethyl) derivatives 1 e, 1 i, 1 j, 3-H and 5 were found to

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